Rabbit specific HRP/AEC IHC Detection Kit - Micro-polymer (ab236468)
Overview
-
Product name
Rabbit specific HRP/AEC IHC Detection Kit - Micro-polymer
See all HRP/AEC Detection Kit kits -
Detection method
Colorimetric -
Assay type
Micro-Polymer -
Product overview
This IHC detection kit is used to detect rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
It is an exact copy of EXPOSE Rabbit specific HRP-AEC detection IHC Kit ab94361, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and AEC chromogen.
-
Notes
For alternative kits, see Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467, and HRP-DAB micro-polymer IHC kits.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
-
Tested applications
Suitable for: IHC-Pmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml AEC Single Solution 1 x 15ml Goat anti-rabbit HRP Conjugate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml -
Research areas
-
Relevance
An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate. -
Alternative names
- carbazole
- Horseradish Peroxidase
Associated products
-
Immunizing Peptide (Blocking)
-
Immunohistochemistry reagents
-
Substrate reagent
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab236468 has been referenced in 2 publications.
- Remic T et al. Development of Tumor Cell-Based Vaccine with IL-12 Gene Electrotransfer as Adjuvant. Vaccines (Basel) 8:N/A (2020). PubMed: 32121641
- Kranjc Brezar S et al. Intratumoral Gene Electrotransfer of Plasmid DNA Encoding shRNA against Melanoma Cell Adhesion Molecule Radiosensitizes Tumors by Antivascular Effects and Activation of an Immune Response. Vaccines (Basel) 8:N/A (2020). PubMed: 32204304