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Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (17)

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Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • ab209101 Melanoma
  • ab209101 Tonsil

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Overview

  • Product name

    Rabbit specific IHC polymer detection kit HRP/DAB
  • Product overview

    Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy. Signal amplification is achieved using an indirect polymer detection system. As Rabbit specific IHC polymer detection kit HRP/DAB does not use avidin or biotin, nonspecific staining from endogenous avidin-biotin activity is eliminated and there is no need for additional steps to block endogenous biotin. The primary antibody specific to an antigen on the tissue section is detected by primary antibody Amplifier. The HRP polymer is added to enable detection with signal amplification. Finally, antibody-bound antigen sites are visualized by adding an appropriate substrate/chromogen (not provided).

  • Tested applications

    Suitable for: IHCmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    Amplifier 1 x 15ml
    Detector 1 x 15ml

Associated products

  • Related Products

    • Anti-PD-L1 antibody [28-8] (ab205921)
    • Universal HIER antigen retrieval reagent (10X) (ab208572)
    • 25x PBS Buffer pH 7.6 (ab64026)
    • Mounting Medium for IHC - Aqueous (ab64230)
    • DAB Substrate Kit (ab64238)
    • Normal Goat Serum (ab7481)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC
Use at an assay dependent concentration.
Notes
IHC
Use at an assay dependent concentration.

Images

  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling USP22 with ab195289 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebrum. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling USP22 with ab195289 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebrum. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling USP22 with ab195289 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human breast carcinoma. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling USP22 with ab195289 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human cerebrum. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling HSF1 with ab52757 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse colon. The section was incubated with ab52757 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling HSF1 with ab52757 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis. The section was incubated with ab52757 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • ab209101 Melanoma
    ab209101 Melanoma

    Immunohistochemistry of human melanoma tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • ab209101 Tonsil
    ab209101 Tonsil

    Immunohistochemistry of human tonsil tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (17)

Publishing research using ab209101? Please let us know so that we can cite the reference in this datasheet.

ab209101 has been referenced in 17 publications.

  • Wei S  et al. Losartan Attenuates Atherosclerosis in Uremic Mice by Regulating Treg/Th17 Balance via Mediating PTEN/PI3K/Akt Pathway. Nephron 146:528-538 (2022). PubMed: 35176745
  • Kudinov VA  et al. Inhaled Placental Mesenchymal Stromal Cell Secretome from Two- and Three-Dimensional Cell Cultures Promotes Survival and Regeneration in Acute Lung Injury Model in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35408778
  • Liu Q  et al. Immuno-PET imaging of PD-L1 expression in patient-derived lung cancer xenografts with [68Ga]Ga-NOTA-Nb109. Quant Imaging Med Surg 12:3300-3313 (2022). PubMed: 35655844
  • Wang H  et al. The Clinical Significance of Deglycosylated PD-L1 Level Detection Using 28-8 Monoclonal Antibody in Lung Adenocarcinoma. Int J Gen Med 15:7383-7393 (2022). PubMed: 36164284
  • Erkhem-Ochir B  et al. Inflammatory and immune checkpoint markers are associated with the severity of aortic stenosis. JTCVS Open 5:1-12 (2021). PubMed: 36003161
View all Publications for this product

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