Cytokine Array – Human Cytokine Antibody Array (Membrane, 42 Targets) (ab133997)
Overview
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Product name
Cytokine Array – Human Cytokine Antibody Array (Membrane, 42 Targets)
See all Cytokine Antibody Array antibody arrays -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human -
Product overview
ab133997 is a cytokine array to be used for the simultaneous detection of 42 Human cytokines. It is suitable for all sample types.
Targets: ENA-78, GCSF, GM-CSF, GRO, GRO-alpha, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1, MCP-2, MCP-3, MCSF, MDC, MIG, MIP-1delta, RANTES, SCF, SDF-1, TARC, TGF-beta1, TNF-alpha, TNF-beta, EGF, IGF-I, Angiogenin, Oncostatin M, Thrombopoietin, VEGF-A, PDGF BB, Leptin
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about cytokine arrays and other membrane antibody arrays
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Notes
If you are interested in this cytokine array, cytokine array ab133998, ab133996, ab169817, ab134003, ab134000, ab169804 and ab169805 may also be of interest.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials Cytokine Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
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| Notes |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
Images
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Multiplex Protein Detection - Human Cytokine Array - Membrane Antibody Array
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.
Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control.
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Cytokine array testing image - AbreviewThis image is courtesy of an anonymous Abreview.The antibody array was used to determine the change in expression of cytokines related to cell proliferation.
One million Human breast cancer cells (MCF7) were cultured in a 10mm dish for 48 hours (in 8ml of RPMI-1640 medium) and were then treated with 5 mM Compound X (not disclosed) for 48 hours. The Abcam protocol was followed for the rest of the procedure. 1 mL of supernatant of culture medium was used for sample incubation (4°C overnight).
Rating: 4.5/5
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Quantification of Human Cytokine Array (membrane antibody array)Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.
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Multiplex Protein Detection - Human Cytokine Array - (membrane antibody array) (ab133997)Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997.
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Quantification of Human Cytokine Array (membrane antibody array)Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997. Mean pixel density was quantified using CCD camera software analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab133997 has been referenced in 15 publications.
- Chiloiro S et al. Markers of humoral and cell-mediated immune response in primary autoimmune hypophysitis: a pilot study. Endocrine 73:308-315 (2021). PubMed: 33484410
- Pantazopoulou V et al. Hypoxia-Induced Reactivity of Tumor-Associated Astrocytes Affects Glioma Cell Properties. Cells 10:N/A (2021). PubMed: 33802060
- Sebastião MJ et al. Bioreactor-based 3D human myocardial ischemia/reperfusion in vitro model: a novel tool to unveil key paracrine factors upon acute myocardial infarction. Transl Res 215:57-74 (2020). PubMed: 31541616
- Lo Re O et al. Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4+CD25+FoxP3+ regulatory T cells activation. Theranostics 10:910-924 (2020). PubMed: 31903159
- Belinová T et al. Immunomodulatory Potential of Differently-Terminated Ultra-Small Silicon Carbide Nanoparticles. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32235697