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    products/immunoassays/human-inflammation-antibody-array-membrane-20-targets-ab169805.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Human Inflammation Antibody Array - Membrane (20 targets) (ab169805)

  • Datasheet
  • SDS
  • Protocol Booklet
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Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169805)

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    Overview

    • Product name

      Human Inflammation Antibody Array - Membrane (20 targets)
      See all Inflammation Antibody Array antibody arrays
    • Sample type

      Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
    • Assay type

      Semi-quantitative
    • Species reactivity

      Reacts with: Human
    • Product overview

      ab169805 detects 20 Human Inflammatory Factors in 4 or 8 samples. Suitable for all liquid sample types.


      Targets: ICAM-1, IL-6 sR, IL-15, IL-16, IL-17, IP-10, MCP-1, MCP-2, M-CSF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, RANTES, TGF beta 1, TNF alpha, TNF beta, sTNFRI, sTNFRII, PDGF-BB


      Learn more about membrane antibody arrays


       

    • Notes

      If you are interested in this cytokine array, arrays ab133997, ab169817, ab133998 and ab134003 may also be of interest. A list of human antibody membrane arrays is available here.

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Tested applications

      Suitable for: Multiplex Protein Detectionmore details

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 4 Membranes 1 x 8 Membranes
      1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl
      1X Blocking Buffer 1 x 25ml 2 x 25ml
      2000X Biotin-Conjugated Anti Cytokines 2 vials 4 vials
      20X Wash Buffer I 1 x 10ml 1 x 20ml
      20X Wash Buffer II 1 x 10ml 1 x 20ml
      2X Cell Lysis Buffer 1 x 10ml 1 x 16ml
      8-Well Incubation Tray (with Lid) 1 unit 1 unit
      Detection Buffer C 1 x 1.5ml 1 x 2.5ml
      Detection Buffer D 1 x 1.5ml 1 x 2.5ml
      Inflammation Antibody Array Membranes 4 units 8 units
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interferons
      • Immunology
      • Innate Immunity
      • Chemokines
      • Beta Chemokines (CC)
      • Antibody Arrays
      • Membrane antibody arrays
      • Inflammation antibody arrays

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab169805 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.
    Notes
    Multiplex Protein Detection
    Use at an assay dependent concentration.

    Images

    • Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169805)
      Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169805)
      Typical results obtained with Abcam Membrane Antibody Arrays. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

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