Mouse Angiogenesis Antibody Array - Membrane (24 Targets) (ab139697)
Overview
-
Product name
Mouse Angiogenesis Antibody Array - Membrane (24 Targets)
See all Angiogenesis Antibody Array antibody arrays -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Mouse -
Product overview
ab139697 is for simultaneous detection of 24 Mouse Angiogenic factors. Suitable for all sample types.
Targets: Eotaxin, Fas Ligand, bFGF, G-CSF, GM-CSF, IFN-gamma, IGF-II, IL-1 alpha, IL-1 beta, IL-12 p40/p70, IL-12 p70, IL-13, IL-6, IL-9, Leptin, MCP-1, M-CSF, MIG, PF-4, TIMP-1, TIMP-2, TNF-alpha, Thrombopoietin.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
-
Notes
A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Angiogenesis Antibody Array Membranes 4 units 8 units Biotin-Conjugated Anti-Cytokines 2 vials 4 vials Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Multiplex Protein Detection
Use at an assay dependent concentration. |
Images
-
Multiplex Protein Detection - Mouse Angiogenesis Antibody Array - Membrane (24 Targets) (ab139697)Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab139697 has been referenced in 3 publications.
- Lee J et al. Melanoma topology reveals a stem-like phenotype that promotes angiogenesis. Sci Adv 3:e1701350 (2017). PubMed: 29075670
- Hedhli J et al. Multimodal Assessment of Mesenchymal Stem Cell Therapy for Diabetic Vascular Complications. Theranostics 7:3876-3888 (2017). PubMed: 29109784
- Khabbazi S et al. Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro. Sci Rep 6:31572 (2016). PubMed: 27514308