For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/lateral-flow/complex-i-human-protein-quantity-dipstick-assay-kit-ab109722.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Mitochondrial
Share by email

Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (8)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
  • ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
  • Sandwich ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)

You may also be interested in

Primary
Product image
Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
Primary
Product image
Anti-MT-ND1 antibody [EPR13466(2)] (ab181848)
Assay
Product image
Mitochondrial Superoxide Assay Kit (Fluorometric) (ab219943)

View more associated products

Overview

  • Product name

    Complex I Human Protein Quantity Dipstick Assay Kit
    See all Complex I kits
  • Sample type

    Cell culture extracts, Tissue
  • Assay type

    Sandwich (quantitative)
  • Species reactivity

    Reacts with: Cow, Human
  • Product overview

    ab109722 contains 48 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex in human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

    Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the Complex I enzyme complex. One antibody is immobilized on the nitrocellulose membrane in a thin line perpendicular to the length of the dipstick, while the other is gold-conjugated and combined with the sample mix. The sample contents containing the gold-conjugated mAb wick past the mAb immobilized on the dipstick. When assembled Complex I is present in the sample, a red line appears at the site of the anti-Complex I antibody line. The signal intensity is directly related to the amount of Complex I in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system. To identify defects in Complex I that do not affect enzyme assembly combine this assay with ab109720 to determine the relative specific activity of Complex I.

  • Notes

    All components are stable in their provided containers at room temperature out of direct sunlight.

    After diluting the 10X Blocking Buffer to 2X, store at 4°C. For long-term storage, all buffers can be stored at 4°C.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 48 tests
    Buffer B (10X Blocking solution) 1 x 0.4ml
    Dipsticks 1 x 30 units
    Extraction Buffer (ab260490) 1 x 15ml
    Gold-conjugated antibody (dried in microplate wells) 1 x 30 tests
    Wash buffer 1 x 2ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
  • Alternative names

    • NADH dehydrogenase

Associated products

  • Related Products

    • Complex I Enzyme Activity Dipstick Assay Kit (ab109720)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent dilution.
Notes
Sandwich ELISA
Use at an assay dependent dilution.

Images

  • ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
    ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
    Figure 2. An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.
  • ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
    ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
    Figure 1. An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.
  • Sandwich ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
    Sandwich ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
    Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab109722? Please let us know so that we can cite the reference in this datasheet.

ab109722 has been referenced in 8 publications.

  • Machado AM  et al. Helicobacter pylori infection affects mitochondrial function and DNA repair, thus, mediating genetic instability in gastric cells. Mech Ageing Dev N/A:N/A (2013). PubMed: 24012633
  • Ceusters JD  et al. Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts. Res Vet Sci 95:870-8 (2013). Functional Studies ; Horse . PubMed: 24099743
  • Pennington K  et al. Differential effects of wild-type and A53T mutant isoform of alpha-synuclein on the mitochondrial proteome of differentiated SH-SY5Y cells. J Proteome Res 9:2390-401 (2010). PubMed: 20334438
  • Ye H  et al. Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts. J Clin Invest 120:1749-61 (2010). PubMed: 20364084
  • Kashimshetty R  et al. Underlying mitochondrial dysfunction triggers flutamide-induced oxidative liver injury in a mouse model of idiosyncratic drug toxicity. Toxicol Appl Pharmacol 238:150-9 (2009). PubMed: 19442681
  • Nadanaciva S  et al. Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis. J Immunol Methods 343:1-12 (2009). PubMed: 19152798
  • Vercauteren K  et al. Short hairpin RNA-mediated silencing of PRC (PGC-1-related coactivator) results in a severe respiratory chain deficiency associated with the proliferation of aberrant mitochondria. J Biol Chem 284:2307-19 (2009). PubMed: 19036724
  • Shikuma CM  et al. Mitochondrial oxidative phosphorylation protein levels in peripheral blood mononuclear cells correlate with levels in subcutaneous adipose tissue within samples differing by HIV and lipoatrophy status. AIDS Res Hum Retroviruses 24:1255-62 (2008). PubMed: 18844460

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

I purchased MSF31 Dipstick assay kit to determine frataxin concentration in whole blood. I wonder if you have any recommendations regarding blood samples collections in EDTA tubes ( K2E K2EDTA, K3E K3EDTA or K2E K2EDTA Sep from Vacuette or other supplier).

Will the results be affected if blood is at room temperature 1 to 2 days prior to freezing? May I add protease inhibitor after thawing or before freezing?

Or will you recommend to add protease inhibitor then freeze it immediatly after collection?

Read More

Abcam community

Verified customer

Asked on Aug 16 2013

Answer



We don’t have a lot of experience with blood samples and Dipstick assays. From the limited experience we have, I suggest to always run on fresh EDTA blood (store at 4C for no more than 1 week). If this is not possible, I suggest to freeze blood viable by adding 10% DMSO, transfer cryovial to a container that would allow cooling of 1C/min until reaching -80C then transfer to vapor phase nitrogen (same way you would do with any cell line) as this will prevent breakage of neutrophils which have a high content of proteases. I have attached a paper showing the process of freezing viable blood.

We have performed the assay here with blood collected on EDTA tubes in collaboration with a local hospital during a clinical trial we run a few years back. Unfortunately I don’t know the name of the supplier they used for the vacutainers.

I don’t know if the results will be affected with if blood is kept at RT. I do not recommend to freeze blood without the DMSO as we have seen a significant decrease in signal with each freeze/thaw cycle (Signal drops even from the first freeze/thaw cycle). Having said that, the group led by David Lynch simply froze the blood without the DMSO and published their data using our frataxin dipsticks http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996613/. As you will see in their paper, we recommend to extract whole blood samples with the extraction buffer provided at a 3:1 ratio (i.e. 900ul of extraction buffer – 300uL of whole blood). After extraction samples must be processed according to the protocol and assay must be carried out with the supernatant. His group has a lot of experience collecting blood and processing for frataxin studies with our dipsticks.

Protease inhibitors may be added before freezing. We have not seen improvement of the signal by adding protease inhibitors after freezing.

Read More

Anja Hoffmann

Abcam Scientific Support

Answered on Aug 16 2013

Question

Hi, I'm looking to assay three cell types for mitochondrial protein quantity and activity. I would like to use the dipstick assays by MitoSciences (ab109720, ab109876, ab109722, and ab109877) but really do not need 30 tests of each--only 3. Is there an "assorted" pack I could purchase that just has one (or 5, or 10) of each of the four assays that are offered?

Read More

Abcam community

Verified customer

Asked on Aug 02 2012

Answer

Thank you for contacting us.

We don't have exactly what you're looking for unfortunately, but we do have MetaPath™ Mito Disease 4-Plex Dipstick Array, ab109879.

The ab109879 is a novel array that allows for the simultaneous quantification of 4 key enzymes whose expression is down-regulated in different mitochondrial diseases: Complex I, Complex IV, PDH and Frataxin. The array is comprised of 4 monoclonal capture antibodies striped onto a dipstick, and the immunocaptured sample is detected using 4 monoclonal detector antibodies that recognize different epitopes on the target enzymes. This array is very rapid (total assay time including sample prep is less than 1 hour) and very sensitive, and as with all Abcam's assays, isolation of mitochondria is not required.

This array is suitable for testing a variety of sample types. Not only is it useful for measuring samples that are deficient in the 4 enzymes due to genetic mutations, it is also useful for measuring changes in the expression of the enzymes due to drug treatments or other manipulations.

https://www.abcam.com/MetaPath™-Mito-Disease-4-Plex-Dipstick-Array-ab109879.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Aug 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.