Pyruvate dehydrogenase (PDH) Protein Quantity Dipstick Assay Kit (ab109883)

Thank you for contacting us yesterday in regards to the Frataxin and Pyruvate dehydrogenase Quantity Dipstick kits (ab109881 and ab109883 respectively).

As discussed over the phone, I contacted MitoSciences to discuss if the kits would be compatible with the experiment you are hoping to perform and if this would be a valuable way of normalising the Frataxin values collected.

I have the following information to share:

The buffer A from ab109881 kitis the same component as thebuffer A from the ab109883 kit. The samples prepared by eluting with this buffer should therefore be compatible for measurement using either kit.

In regards to the questions whether the Pyruvate dehydrogenase kit is likely to serve as a good normalising standard of your samples this is a less straight forward answer. The PHD Protein Quantity Kit (ab109883) will allow you to measure the PHD quantity present in yoursampleswith sufficient accuracy to use as a normalising value. However,even though Pyruvate dehydrogenase is ubiquitous andtheproteinisencoded by “housekeeping genes”, deciding on the perfect control will vary a lot on the population you are looking at.

It would be worthwhile to testwhether or not PDH is a good control protein for your particular sample. One way of doing this is by doing a longitudinal study on a small control cohort to determine: (1) Does PDH change significantly from subject to subject and (2) does PDH change significantly through time in the same cohort.If the answer to both is NO, then it could be used as a control protein.





I hope this has sufficiently answered your questions but please do not hesitate to contact us again if you have any further questions.

Thank you for your enquiry.

For the sandwich ELISA dipstick ab109881, the buffers are the same. However for the PDH activity dipstick ab109883 they are very different.

I am sorry that as we have over 80 0000 products it is not possible to send test samples. I would like to reassure you that we can provide our 6 months guarantee for products used in the tested applications and species specified on the individual datasheets. If they do not work in the tested applications and species, we can provide a refund, credit note or free of charge replacement.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Thank you for contacting us.

Regarding ab110172 I received the following information from the lab:

This kit can be used to test serum samples. Our suggestion for the customer would be to spike a control serum sample with pure cytochrome c to use as a control. Cytochrome c and other mitochondrial proteins are only found in the serum when leackage due to periphereal cell damage occurs (i.e liver damage, muscle damage).

We know of a customer who has successfully used ab109883 to quantify PDH in plasma following presumably severe tissue damage. The data was shown to us in confidentiality and therefore we cannot share any results obtained. But the kit is certainly sensitive enough to detect small amount of leakage from tissues onto serum.

There is a growing literature in regards to leakage of mitochondrial proteins in plasma due to systemic inflammatory response syndrome, influenza-associated encephalopathy, fulminant hepatitis, and reperfusion injury.



To find other kits and products for mitochondrial proteins, please check out the following links for further information:

https://www.abcam/com/metabolismand then the dropdown menu on the left called "Mitochondrial Metabolism"

https://www.abcam.com/index.html?pageconfig=resource&rid=13854

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.