For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/panels/cell-cycle-and-apoptosis-wb-cocktail-pcdkphh3actinparp-ab139417.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Antibody Cocktails Apoptosis & Cell Cycle
Share by email

Cell Cycle and Apoptosis WB Cocktail (pCdk/pHH3/Actin/PARP) (ab139417)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot – Cell Cycle + Apoptosis Western Blot Cocktail (ab139417):  HeLa cells
  • Western blot – Cell Cycle and Apoptosis Western Blot Cocktail  (ab139417):  Jurkat cells

You may also be interested in

Primary
Product image
Anti-Cyclin E1 antibody [EPR194] (ab133266)
Cocktail
Product image
Oxidative Stress Defense (Catalase, SOD1, TRX, smooth muscle Actin) Western Blot Cocktail (ab179843)
Primary
Product image
Anti-Cleaved PARP1 antibody (ab4830)

View more associated products

Overview

  • Product name

    Cell Cycle and Apoptosis WB Cocktail (pCdk/pHH3/Actin/PARP)
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Cell Cycle & apoptosis western blot cocktail (ab139417) is designed to provide a rapid assessment of cell cycle distribution and apoptosis induction based on molecular markers for the G1/S and M phases of the cell cycle and the presence of cleaved PARP. This is a mixture of four highly specific monoclonal primary antibodies targeting Cdk2 pTyr15, Histone H3 pSer10 , cleaved-PARP and Actin. Cyclin-dependent kinase 2 (Cdk2) is maintained in an inactive state in G1/S by inhibitory phosphorylation on Tyr15. Histone H3 is phosphorylated at Ser10 when chromosomes condense during mitosis. Hence, elevated Cdk2 pTyr15 indicates G1/S arrested cells and elevated Histone H3 pSer10 indicates M-phase arrested cells when compared to asynchronous cycling control cells. Poly [ADP-ribose] polymerase 1 (PARP) is a DNA repair enzyme that is cleaved by activated caspases, hence cleaved-PARP indicates induction of apoptosis. An anti- Actin antibody is included as a loading control. These four readouts are easily resolved by western blot given their different molecular weights. Because this western blot cocktail is a mix of rabbit and mouse monoclonal primary antibodies, a secondary antibody cocktail of HRP conjugated antibodies (anti-mouse and anti-rabbit) is provided.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 200 µl
    2500X HRP Conjugated Secondary Antibody Cocktail 1 x 50µl
    250X Cell Cycle & Apoptosis WB Cocktail 1 x 200µl
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Antibody Cocktails
    • Apoptosis & Cell Cycle
  • Alternative names

    • ACTC
    • ACTC1
    • Actin alpha cardiac muscle 1
    • ADP ribosyltransferase diphtheria toxin like 1
    • ADPRT 1
    • Alpha cardiac actin
    • ARTD1
    • CDK2
    • CDKN2
    • Cell division protein kinase 2
    • Cyclin dependent kinase 2
    • H3/g
    • H3/t
    • H3FT
    • H3t
    • HIST3H3
    • Histone H3.1t
    • NAD(+) ADP ribosyltransferase 1
    • p33 protein kinase
    • PARP 1
    • PARP1
    • Poly [ADP-ribose] polymerase 1
    • Poly[ADP ribose] synthase 1
    see all
  • Database links

    • Entrez Gene: 142 Human
    • Entrez Gene: 1017 Human
    • Entrez Gene: 70 Human
    • Entrez Gene: 8290 Human
    • Omim: 173870 Human
    • Omim: 116953 Human
    • Omim: 102540 Human
    • Omim: 602820 Human
    • SwissProt: P68032 Human
    • SwissProt: P24941 Human
    • SwissProt: Q16695 Human
    • SwissProt: P09874 Human
    see all

Associated products

  • Related Products

    • Cell Cycle (pCdk/pHH3/Actin) WB Cocktail (ab136810)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.

1/250 dilution for primary antibody cocktail.

1/2500 dilution for secondary antibody HRP cocktail.

Notes
WB
Use at an assay dependent concentration.

1/250 dilution for primary antibody cocktail.

1/2500 dilution for secondary antibody HRP cocktail.

Images

  • Western blot – Cell Cycle + Apoptosis Western Blot Cocktail (ab139417):  HeLa cells
    Western blot – Cell Cycle + Apoptosis Western Blot Cocktail (ab139417): HeLa cells
    Paclitaxel induces dose-dependent G2/M-phase arrest and induces apoptosis (decreased Cdk2 pTyr15, increased Histone H3 pSer10, cleaved PARP present).

    Primary antibody: All lanes Cell Cycle WB cocktail (MS1123) diluted to 1X in 4% milk/PBS.

    Lane 1: HeLa lysate; Untreated, asynchronous cells
    Lane 2: HeLa lysate; 2000 nM Paclitaxel, 24h
    Lane 3: HeLa lysate; 100 nM Paclitaxel, 24h
    Lane 4: HeLa lysate; 5 nM Paclitaxel, 24h
    Lane 5: HeLa lysate; 0.25 nM Paclitaxel, 24h

    All lysates at 15 µg per lane. Lysates are HeLa cells lysed in SDS lysis buffer.

    Secondary antibody: All lanes are the WB secondary antibody HRP cocktail diluted to 1X in 4% milk/PBS.
  • Western blot – Cell Cycle and Apoptosis Western Blot Cocktail  (ab139417):  Jurkat cells
    Western blot – Cell Cycle and Apoptosis Western Blot Cocktail (ab139417): Jurkat cells
    Hydroxyurea induces a G1/S-phase cell cycle arrest and induces apoptosis (increased Cdk2 pTyr15, decreased Histone H3 pSer10, cleaved PARP present). Paclitaxel induces dose-dependent G2/M-phase arrest and induces apoptosis (decreased Cdk2 pTyr15, increased Histone H3 pSer10, cleaved PARP present).

    Primary antibody: All lanes Cell Cycle WB cocktail (ab139417) diluted to 1X in 4% milk/PBS.

    Lane 1: Jurkat lysate; Untreated, asynchronous cells
    Lane 2: Jurkat lysate; 5 mM Hydroxyurea, 24h
    Lane 3: Jurkat lysate; 1000 nM Paclitaxel, 24h
    Lane 4: Jurkat lysate; 50 nM Paclitaxel, 24h
    Lane 5: Jurkat lysate; 2.5 nM Paclitaxel, 24h

    All lysates at 12 µg per lane. Lysates are Jurkat cells lysed in SDS lysis buffer.

    Secondary antibody: All lanes are the WB secondary antibody HRP cocktail diluted to 1X in 4% milk/PBS.

    Predicted & observed band size: 89, 42, 33, 17 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab139417? Please let us know so that we can cite the reference in this datasheet.

ab139417 has been referenced in 2 publications.

  • Wang B  et al. Combination of HDE and BIIB021 efficiently inhibits cell proliferation and induces apoptosis via downregulating hTERT in myelodysplastic syndromes. Exp Ther Med 21:503 (2021). PubMed: 33791012
  • Robertson D  et al. A community-based continuing care program for the elderly disabled: an evaluation of planned intermittent hospital readmission. J Gerontol 32:334-9 (1977). IHC . PubMed: 139417

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab139417.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.