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Signal Transduction Metabolism Mitochondrial
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Complex II WB Antibody Cocktail (ab110410)

  • Datasheet
  • SDS
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Western blot - Complex II WB Antibody Cocktail (ab110410)

    Key features and details

    • Assay type: Quantitative

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    Overview

    • Product name

      Complex II WB Antibody Cocktail
      See all Complex II kits
    • Assay type

      Quantitative
    • Species reactivity

      Reacts with: Mouse, Rat, Cow, Human
    • Product overview

      Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).


      Cocktail Antibodies:


      Mouse C-V-alpha [15H4C4] monoclonal:


      Amount: 50 µg


      Working concentration: 1 µg/ml


      Mouse C-II-30 (FeS) [21A11AE7] monoclonal:


      Amount: 250 µg


      Working concentration: 5 µg/ml


      Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:


      Amount: 5 µg


      Working concentration: 0.1 µg/ml


       

    • Notes

      Related products

      Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

    • Tested applications

      Suitable for: WBmore details

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 305 µg
      Pre-mixed solution of 3 monoclonal antibodies 1 x 305µg
    • Research areas

      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Other Metabolism Assay
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Kits/ Lysates/ Other
      • Kits
      • Antibody Cocktails
      • OXPHOS
    • Relevance

      Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
    • Alternative names

      • SDH
      • Succinate coenzyme Q reductase
      • Succinate dehydrogenase
    • Database links

      • Entrez Gene: 6390 Human
      • Entrez Gene: 6389 Human
      • Entrez Gene: 6391 Human
      • SwissProt: P31040 Human
      • SwissProt: P21912 Human
      • SwissProt: Q99643 Human
      • SwissProt: O14521 Human
      • SwissProt: 6392 Human

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab110410 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      WB
      Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.
      Notes
      WB
      Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.

      Images

      • Western blot - Complex II WB Antibody Cocktail (ab110410)
        Western blot - Complex II WB Antibody Cocktail (ab110410)
        All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution

        Lane 1 : human heart mitochondria at 10 µg
        Lane 2 : Immunoprecipitated Complex II at 0.5 µg


        The Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa) and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (29)

      Publishing research using ab110410? Please let us know so that we can cite the reference in this datasheet.

      ab110410 has been referenced in 29 publications.

      • Zheng Z  et al. NR4A1 promotes TNF-a-induced chondrocyte death and migration injury via activating the AMPK/Drp1/mitochondrial fission pathway. Int J Mol Med 45:151-161 (2020). PubMed: 31746366
      • Wu K  et al. Cigarette smoke extract increases mitochondrial membrane permeability through activation of adenine nucleotide translocator (ANT) in lung epithelial cells. Biochem Biophys Res Commun 525:733-739 (2020). PubMed: 32143825
      • Zhao S  et al. Nurr1 Promotes Lung Cancer Apoptosis Via Enhancing Mitochondrial Stress and p53-Drp1 Pathway. Open Life Sci 14:262-274 (2019). PubMed: 33817160
      • Zhang L  et al. Anti-tumor effect of LATS2 on liver cancer death: Role of DRP1-mediated mitochondrial division and the Wnt/ß-catenin pathway. Biomed Pharmacother 114:108825 (2019). PubMed: 30981110
      • Zhang Y  et al. Matrine promotes apoptosis in SW480 colorectal cancer cells via elevating MIEF1-related mitochondrial division in a manner dependent on LATS2-Hippo pathway. J Cell Physiol 234:22731-22741 (2019). PubMed: 31119752
      View all Publications for this product

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