Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Cytochrome c Apoptosis WB Antibody Cocktail
See all ApoTrack™ Cytochrome c Apoptosis kits -
Assay type
Quantitative -
Species reactivity
Reacts with: Human -
Product overview
The permeabilization of mitochondrial outer membrane and the subsequent release of cytochrome c and other apoptogenic proteins from mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest for many researchers.
ab 110415 (MSA12) is a Western blot antibody cocktail that allows for the detection of cytochrome c in cytoplasmic and mitochondria-containing fractions for determining the proportion of released cytochrome c from mitochondria to the cytoplasm from apoptosis. The kit includes antibodies against a cytoplasmic protein marker, glyceraldehyde-3-phosphodehydrogenase (GAPDH), and 2 mitochondrial markers, pyruvate dehydrogenase subunit E1-alpha (a matrix marker), and ATP synthase subunit alpha (an inner membrane marker). This set of control markers allows for the monitoring and/or optimization of the permeabilization conditions.
Cocktail Antibodies:
Mouse anti Cyt. c monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse anti GAPDH monclonal:
Amount: 5ug
Working concentration: 0.1 µg/ml
Mouse anti PDH-E1-alpha monoclonal:
Amount: 100ug
Working concentration: 2 µg/ml
Mouse anti C-V-alpha monoclonal:
Amount: 25ug
Working concentration: 0.5 µg/ml
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Notes
This product was previously called ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail
Other apoptosis assays
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
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Tested applications
Suitable for: WBmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 180 µg HeLa Lysate Control 1 x 50µg Pre-mixed Solution of 4 monoclonal Antibodies 1 x 180µg -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 3.6 µg/ml. The antibody cocktail (0.9 mg/ml) should be diluted 250x to a final working concentration of 3.6 µg/ml for Western blotting.
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| Notes |
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WB
Use a concentration of 3.6 µg/ml. The antibody cocktail (0.9 mg/ml) should be diluted 250x to a final working concentration of 3.6 µg/ml for Western blotting. |
Images
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Western blot - ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit ab109719/MS861) and probed using ab110415 (MSA12). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab110415 has been referenced in 9 publications.
- Kurppa KJ et al. Treatment-Induced Tumor Dormancy through YAP-Mediated Transcriptional Reprogramming of the Apoptotic Pathway. Cancer Cell 37:104-122.e12 (2020). PubMed: 31935369
- Tran LNK et al. The Combination of Metformin and Valproic Acid Induces Synergistic Apoptosis in the Presence of p53 and Androgen Signaling in Prostate Cancer. Mol Cancer Ther 16:2689-2700 (2017). PubMed: 28802253
- Deuse T et al. Dichloroacetate prevents restenosis in preclinical animal models of vessel injury. Nature 509:641-4 (2014). PubMed: 24747400
- Estlack LE et al. Nanosecond pulsed electric fields modulate the expression of Fas/CD95 death receptor pathway regulators in U937 and Jurkat Cells. Apoptosis 19:1755-68 (2014). PubMed: 25331537
- Burchell VS et al. The Parkinson's disease-linked proteins Fbxo7 and Parkin interact to mediate mitophagy. Nat Neurosci 16:1257-65 (2013). PubMed: 23933751
- Alquezar C et al. Inactivation of CDK/pRb pathway normalizes survival pattern of lymphoblasts expressing the FTLD-progranulin mutation c.709-1G>A. PLoS One 7:e37057 (2012). PubMed: 22623979
- Deas E et al. PINK1 cleavage at position A103 by the mitochondrial protease PARL. Hum Mol Genet 20:867-79 (2011). WB . PubMed: 21138942
- Plant PJ et al. Absence of caspase-3 protects against denervation-induced skeletal muscle atrophy. J Appl Physiol 107:224-34 (2009). PubMed: 19390003
- Arnold M [Value of chlorhexidine in combined prophylactic and therapeutic measures]. Zahnarztl Prax 40:10-3 (1989). PubMed: 2623979