For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/panels/endoplasmic-reticulum-fraction-western-blot-cocktail-ab139415.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neurodegenerative disease Huntington's disease
Share by email

Endoplasmic Reticulum Fraction Western Blot Cocktail (ab139415)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Component Separation
  • Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Cell Fractionation
  • Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Cross Reactivity

Key features and details

  • Sample type: Cell Lysate, Nuclear Extracts, Tissue Extracts, Tissue Homogenate

You may also be interested in

Cocktail
Product image
Organelle Detection Western Blot Cocktail (ab133989)
ELISA
Product image
Complex I Rodent Profiling ELISA Kit (ab136809)

View more associated products

Overview

  • Product name

    Endoplasmic Reticulum Fraction Western Blot Cocktail
  • Sample type

    Tissue Extracts, Cell Lysate, Tissue Homogenate, Nuclear Extracts
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    ab139415 contains 3 mAbs each targeting a specific organelle marker. The presence of endoplasmic reticulum is detected by GRP78; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9). This cocktail is suitable for determining the purity of organelle isolates prior to further characterization.

    This product is particularly valuable to researchers working in organelle proteomics. Mass spectrometry is frequently used in this field to determine the protein content of targeted organelle isolates. These isolates are obtained using differential centrifugation, density gradient fractionation, biochemical enrichment, or affinity purification. Unfortunately, the various methods of purification available for organelle isolation are imperfect and leave behind contaminants from undesired regions of the cell. These contaminants are inevitable, but being aware of which contaminants are present is crucial for analysis of mass spectrometry results. The high sensitivity and species cross reactivity of the antibodies in this cocktail will quickly and easily reveal impurities caused by imperfect sample preparation.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 200 µl
    2500X HRP Conjugated Secondary Antibody Cocktail 1 x 50µl
    250X Endoplasmic Reticulum Fraction Western Blot Cocktail 1 x 200µl
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Kits/ Lysates/ Other
    • Kits
    • Antibody Cocktails
    • Apoptosis & Cell Cycle
  • Cellular localization

    GRP78 BiP: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. GAPDH: Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. Histone H3: Nucleus. Chromosome.
  • Database links

    • Entrez Gene: 2597 Human
    • Entrez Gene: 3309 Human
    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 100042025 Mouse
    • Entrez Gene: 14433 Mouse
    • Entrez Gene: 14828 Mouse
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 24383 Rat
    • Entrez Gene: 25617 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Entrez Gene: 685186 Rat
    • Omim: 138120 Human
    • Omim: 138400 Human
    • Omim: 602810 Human
    • SwissProt: P04406 Human
    • SwissProt: P11021 Human
    • SwissProt: P68431 Human
    • SwissProt: P16858 Mouse
    • SwissProt: P20029 Mouse
    • SwissProt: P68433 Mouse
    • SwissProt: P04797 Rat
    • SwissProt: P06761 Rat
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 544577 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Unigene: 743241 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 304088 Mouse
    • Unigene: 309092 Mouse
    • Unigene: 317779 Mouse
    • Unigene: 330160 Mouse
    • Unigene: 343110 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 392463 Mouse
    • Unigene: 392480 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 414470 Mouse
    • Unigene: 458138 Mouse
    • Unigene: 458416 Mouse
    • Unigene: 470180 Mouse
    • Unigene: 474909 Mouse
    • Unigene: 475698 Mouse
    • Unigene: 11088 Rat
    • Unigene: 129558 Rat
    • Unigene: 138090 Rat
    • Unigene: 91450 Rat
    see all

Associated products

  • Related Products

    • Mitochondria Fraction Western Blot Cocktail (ab139416)
    • Membrane Fraction WB Cocktail (ab140365)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.

Primary antibody cocktail = 1/250 dilution

Secondary antibody cocktail = 1/2500 dilution

Notes
WB
Use at an assay dependent concentration.

Primary antibody cocktail = 1/250 dilution

Secondary antibody cocktail = 1/2500 dilution

Images

  • Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Component Separation
    Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Component Separation
    Developed using the ECL technique; Performed under reducing conditions; Exposure time: 5 mins. All blocking and antibody incubation steps were performed in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20

    Samples
    Lane 1: Marker
    Lanes 2-5: Hela Whole Cell Lysate – 20 µg

    Primary antibody:
    Lane 1: None
    Lane 2: Anti-GRP78 antibody – Endoplasmic Reticulum Marker
    Lane 3: Anti- GAPDH antibody – Cytosolic Marker
    Lane 4: Anti- Histone H3 (di methyl K9) antibody – Nuclear Marker
    Lane 5: Assembled Endoplasmic Reticulum Antibody Cocktail

    Secondary:HRP conjugated secondary antibody cocktail at 1/2500

    Predicted GRP78 band size: 78 kDa
    Observed band size: 78 kDa

    Predicted GAPDH band size: 37 kDa
    Observed band size: 37 kDa

    Predicted Histone H3 (di methyl K9) band size: 17 kDa
    Observed band size: 17 kDa
  • Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Cell Fractionation
    Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Cell Fractionation
    HeLa cell lysates were prepared using the Membrane Fractionation Kit (ab139409), blots were developed using the ECL technique, performed under reducing conditions and exposed for 5 minutes. A 4-15% gradient acrylamide gel was used and blocking and antibody incubation steps were performed in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20.

    Sample lanes:
    Lane 1 – Marker
    Lane 2 - HeLa whole cell lysate - 20 µL
    Lane 3 - HeLa cytosolic fraction lysate - 20 µL
    Lane 4 - HeLa membrane fraction lysate - 20 µL
    Lane 5 - HeLa nuclear fraction lysate - 20 µL

    Primary antibody: ER Fraction WB cocktail at 1/250 dilution
    Secondary antibody: HRP conjugated secondary cocktail at 1/2500.

    Predicted/observed band sizes:
    GRP78: 78 kDa/78 kDa
    GAPDH: 37 kDa/37 kDa
    Histone H3 (di methyl K9): 17 kDa/17 kDa
  • Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Cross Reactivity
    Western Blot for Endoplasmic Reticulum Membrane Antibody Cocktail – Cross Reactivity
    Developed using the ECL technique, performed under reducing conditions and exposed for 3 minutes. All blocking and antibody incubation steps performed in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20.

    Samples:
    1: Marker
    2: Human heart homogenate lysate - 20 µg
    3: HeLa cell lysate - 20 µg
    4: Mouse heart homogenate lysate - 20 µg
    5: NIH3T3 cell lysate - 20 µg
    6: Rat heart homogenate lysate - 20 µg
    7: H9C2 cell lysate - 20 µg

    Primary antibody:ER Fraction WB cocktail at 1/250.
    Secondary antibody: HRP conjugated secondary antibody cocktail at 1/2500.

    Predicted/Observed band sizes:
    GRP78: 78 kDa/75 kDa
    GAPDH: 37 kDa/37 kDa
    Histone H3 (dimethyl K9): 17 kDa/17 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab139415? Please let us know so that we can cite the reference in this datasheet.

ab139415 has been referenced in 2 publications.

  • Pérez-Rodriguez S  et al. Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis. PLoS One 15:e0237930 (2020). PubMed: 32841274
  • Micakovic T  et al. Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes. Bio Protoc 9:e3379 (2019). PubMed: 33654875

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab139415.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.