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    products/panels/epithelial-mesenchymal-transition-beta-catenin-vimentin-western-blot-cocktail-ab157392.pdf

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Kits/ Lysates/ Other Kits Antibody Cocktails Epithelial-Mesenchymal
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Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail (ab157392)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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ab157392 tested on a panel of cell lines
  • Western blot of ab157392 tested on breast cancer cell lines MCF7 and MDA-MB-231.

Key features and details

  • Sample type: Cell Lysate

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Overview

  • Product name

    Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail
  • Sample type

    Cell Lysate
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Product overview

    This Epithelial-Mesenchymal Transition (EMT) western blot cocktail is designed to determine the relative abundance of canonical EMT markers. EMT is a process in which a cell transitions from an epithelial-like (attached, polarized) state to a mesenchymal-like (unattached, migratory) state and such transitions occur during development and metastasis. Underlying the morphological cell changes during EMT are transcription factor programs, biochemical cascades and alterations to the cytoskeleton. Beta-Catenin is a transcription factor in the Wnt signaling pathway and is involved in the regulation of cell adhesion; beta-Catenin is typically more abundant in epithelial-like cells. Vimentin is an intermediate filament typically found in non-epithelial and mesenchymal cells. Alpha Smooth Muscle Actin and GAPDH are also included in this cocktail as loading controls (Alpha Smooth Muscle Actin is reported to be more highly expressed in mesenchymal-like cells, but we find it to be widely expressed in transformed cell lines).

    These four readouts are easily resolved by western blot given their different molecular weights. Because this western blot cocktail is a mix of rabbit and mouse monoclonal primary antibodies.

    A secondary antibody cocktail of HRP conjugated antibodies (anti-mouse and anti-rabbit) is provided.

    Expected and observed MWs:

    • Beta-Catenin 92kDa
    • Vimentin 54kDa
    • Smooth Muscle Actin 42kDa
    • GAPDH 36kDa

     

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 200 µl
    2500X HRP Conjugated Secondary Antibody Cocktail 1 vial
    250X EMT WB Cocktail 1 vial
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Antibody Cocktails
    • Epithelial-Mesenchymal
  • Alternative names

    • 38 kDa BFA-dependent ADP-ribosylation substrate
    • a actin
    • AAT6
    • ACTA_HUMAN
    • ACTA2
    • Actin alpha 2 smooth muscle aorta
    • Actin aortic smooth muscle
    • Actin, aortic smooth muscle
    • ACTSA
    • ACTVS
    • aging associated gene 9 protein
    • Aging-associated gene 9 protein
    • Alpha 2 actin
    • Alpha actin 2
    • Alpha cardiac actin
    • alpha sma
    • Alpha-actin-2
    • b-catenin
    • BARS-38
    • Beta catenin
    • Beta-catenin
    • Cadherin associated protein
    • Catenin (cadherin associated protein), beta 1, 88kDa
    • Catenin beta 1
    • Catenin beta-1
    • CATNB
    • cb609
    • Cell growth inhibiting gene 46 protein
    • Cell growth-inhibiting gene 46 protein
    • CHBCAT
    • CTNB1_HUMAN
    • CTNNB
    • CTNNB1
    • CTRCT30
    • DKFZp686D02253
    • EC 1.2.1.12
    • Epididymis luminal protein 113
    • Epididymis secretory sperm binding protein Li 162eP
    • FLJ25606
    • FLJ36605
    • FLJ37923
    • G3P_HUMAN
    • G3PD
    • G3PDH
    • GAPD
    • GAPDH
    • GIG46
    • Glyceraldehyde 3 phosphate dehydrogenase
    • glyceraldehyde 3-PDH
    • Glyceraldehyde-3-phosphate dehydrogenase
    • Growth inhibiting gene 46
    • HEL-S-162eP
    • HEL113
    • KNC-NDS6
    • MGC102544
    • MGC102546
    • MGC103190
    • MGC103191
    • MGC105239
    • MGC127711
    • MGC88685
    • MYMY5
    • OCAS, p38 component
    • OCT1 coactivator in S phase, 38-KD component
    • OTTHUMP00000162082
    • OTTHUMP00000165222
    • OTTHUMP00000165223
    • OTTHUMP00000209288
    • OTTHUMP00000209289
    • peptidyl cysteine S nitrosylase GAPDH
    • Peptidyl-cysteine S-nitrosylase GAPDH
    • VIM
    • VIME_HUMAN
    • Vimentin
    • wu:fb33a10
    see all
  • Database links

    • Entrez Gene: 2597 Human
    • Entrez Gene: 24383 Human
    • Entrez Gene: 193060 Human
    • Entrez Gene: 1499 Human
    • Entrez Gene: 59 Human
    • Omim: 138400 Human
    • Omim: 7431 Human
    • Omim: 116806 Human
    • Omim: 102620 Human
    • SwissProt: P35222 Human
    • SwissProt: P08670 Human
    • SwissProt: P62736 Human
    • SwissProt: P04406 Human
    see all

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.

Expected and observed MWs:

Beta-Catenin 92kDa

Vimentin 54kDa

Smooth Muscle Actin 42kDa

GAPDH 36kDa

Notes
WB
Use at an assay dependent concentration.

Expected and observed MWs:

Beta-Catenin 92kDa

Vimentin 54kDa

Smooth Muscle Actin 42kDa

GAPDH 36kDa

Images

  • ab157392 tested on a panel of cell lines
    ab157392 tested on a panel of cell lines
    Primary antibody: all lanes EMT WB cocktail (ab157392) diluted to 1X in 4% milk/PBS.

    Lane 1. MDA-MB-231 cell lysate
    Lane 2. SH SY5Y cell lysate
    Lane 3. HeLa cell lysate
    Lane 4. Jurkat cell lysate.

    All lysates loaded 20 µg per lane. Lysates were generated in RIPA lysis buffer (ab156034) and briefly boiled in sample buffer before loading on SDS-PAGE.

    Secondary antibody: All lanes using the included HRP Conjugated Secondary Antibody Cocktail (included with ab157392) diluted to 1X in 5% milk/PBS.
  • Western blot of ab157392 tested on breast cancer cell lines MCF7 and MDA-MB-231.
    Western blot of ab157392 tested on breast cancer cell lines MCF7 and MDA-MB-231.
    MCF7 is epithelial-like and MDA-MB-231 cells are mesenchymal-like based on the relative expression of beta-Catenin and Vimentin between the cell lines.

    Primary antibody: all lanes EMT WB cocktail diluted to 1X in 5% milk/PBS.

    Lane 1. MCF7 cell lysate
    Lane 2. MDA-MB-231 cell lysate

    All lysates loaded 20 µg per lane. Lysates were generated in SDS lysis buffer and briefly boiled in sample buffer before loading on SDS-PAGE.

    Secondary antibody: All lanes using the included HRP Conjugated Secondary Antibody Cocktail (included with ab157392) diluted to 1X in 5% milk/PBS.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab157392? Please let us know so that we can cite the reference in this datasheet.

ab157392 has been referenced in 1 publication.

  • Matsushima H  et al. Sox9 expression in carcinogenesis and its clinical significance in intrahepatic cholangiocarcinoma. Dig Liver Dis 47:1067-75 (2015). WB . PubMed: 26341967

Customer reviews and Q&As

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Question

Can you please provide some recommendations or references for mouse-derived positive controls for product Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail (ab157392)?

Read More

Abcam community

Verified customer

Asked on Nov 24 2014

Answer

Examples of suitable mouse-derived positive controls for product Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail (ab157392) include spleen cells, lymphocytes and chondrocytes. For reference, please see:

Altered endochondral ossification in collagen X mouse models leads to impaired immune responses. PMCID: PMC2630710.

The mouse collagen X gene: complete nucleotide sequence, exon structure and expression pattern. PMCID: PMC1132157.

Type X collagen gene expression in mouse chondrocytes immortalized by a temperature-sensitive simian virus 40 large tumor antigen. PMCID: PMC2120322.

Read More

Abcam Scientific Support

Answered on Nov 24 2014

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