Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail (ab157392)
Key features and details
- Sample type: Cell Lysate
Overview
-
Product name
Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail -
Sample type
Cell Lysate -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Product overview
This Epithelial-Mesenchymal Transition (EMT) western blot cocktail is designed to determine the relative abundance of canonical EMT markers. EMT is a process in which a cell transitions from an epithelial-like (attached, polarized) state to a mesenchymal-like (unattached, migratory) state and such transitions occur during development and metastasis. Underlying the morphological cell changes during EMT are transcription factor programs, biochemical cascades and alterations to the cytoskeleton. Beta-Catenin is a transcription factor in the Wnt signaling pathway and is involved in the regulation of cell adhesion; beta-Catenin is typically more abundant in epithelial-like cells. Vimentin is an intermediate filament typically found in non-epithelial and mesenchymal cells. Alpha Smooth Muscle Actin and GAPDH are also included in this cocktail as loading controls (Alpha Smooth Muscle Actin is reported to be more highly expressed in mesenchymal-like cells, but we find it to be widely expressed in transformed cell lines).
These four readouts are easily resolved by western blot given their different molecular weights. Because this western blot cocktail is a mix of rabbit and mouse monoclonal primary antibodies.
A secondary antibody cocktail of HRP conjugated antibodies (anti-mouse and anti-rabbit) is provided.
Expected and observed MWs:
- Beta-Catenin 92kDa
- Vimentin 54kDa
- Smooth Muscle Actin 42kDa
- GAPDH 36kDa
-
Tested applications
Suitable for: WBmore details
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 µl 2500X HRP Conjugated Secondary Antibody Cocktail 1 vial 250X EMT WB Cocktail 1 vial -
Research areas
-
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate
- a actin
- AAT6
see all -
Database links
- Entrez Gene: 2597 Human
- Entrez Gene: 24383 Human
- Entrez Gene: 193060 Human
- Entrez Gene: 1499 Human
- Entrez Gene: 59 Human
- Omim: 138400 Human
- Omim: 7431 Human
- Omim: 116806 Human
see all
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
Expected and observed MWs: Beta-Catenin 92kDa Vimentin 54kDa Smooth Muscle Actin 42kDa GAPDH 36kDa |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Expected and observed MWs: Beta-Catenin 92kDa Vimentin 54kDa Smooth Muscle Actin 42kDa GAPDH 36kDa |
Images
-
ab157392 tested on a panel of cell linesPrimary antibody: all lanes EMT WB cocktail (ab157392) diluted to 1X in 4% milk/PBS.
Lane 1. MDA-MB-231 cell lysate
Lane 2. SH SY5Y cell lysate
Lane 3. HeLa cell lysate
Lane 4. Jurkat cell lysate.
All lysates loaded 20 µg per lane. Lysates were generated in RIPA lysis buffer (ab156034) and briefly boiled in sample buffer before loading on SDS-PAGE.
Secondary antibody: All lanes using the included HRP Conjugated Secondary Antibody Cocktail (included with ab157392) diluted to 1X in 5% milk/PBS. -
Western blot of ab157392 tested on breast cancer cell lines MCF7 and MDA-MB-231.MCF7 is epithelial-like and MDA-MB-231 cells are mesenchymal-like based on the relative expression of beta-Catenin and Vimentin between the cell lines.
Primary antibody: all lanes EMT WB cocktail diluted to 1X in 5% milk/PBS.
Lane 1. MCF7 cell lysate
Lane 2. MDA-MB-231 cell lysate
All lysates loaded 20 µg per lane. Lysates were generated in SDS lysis buffer and briefly boiled in sample buffer before loading on SDS-PAGE.
Secondary antibody: All lanes using the included HRP Conjugated Secondary Antibody Cocktail (included with ab157392) diluted to 1X in 5% milk/PBS.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab157392 has been referenced in 1 publication.
- Matsushima H et al. Sox9 expression in carcinogenesis and its clinical significance in intrahepatic cholangiocarcinoma. Dig Liver Dis 47:1067-75 (2015). WB . PubMed: 26341967