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Mesenchymal Stromal Cell Marker (CD44, CD45, CD90 CD29, CD105) Antibody Panel - Human (ab93758)

Submit a review Q&A (6)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)
  • Mesenchymal Stromal Cell Marker (CD44, CD45, CD90 CD29, CD105) Antibody Panel - Human (ab93758)

Overview

  • Product name

    Mesenchymal Stromal Cell Marker (CD44, CD45, CD90 CD29, CD105) Antibody Panel - Human

  • Product overview

    Mesenchymal Stromal Cell Marker (CD44, CD45, CD90 CD29, CD105) Antibody Panel ab93758 contains multiple trial-sized versions of clones against key human mesenchymal stromal cell markers, and includes a CD44 mouse monoclonal, CD45 rabbit polyclonal, CD90 mouse monoclonal, CD29 rabbit monoclonal and CD105 mouse monoclonal antibody.


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


    Anti-CD44 antibody [F10-44-2] (ab6124)


    Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)


    Anti-CD90 / Thy1 antibody [AF-9] (ab23894)


    Anti-CD105 antibody [105C02] (ab44967)


    Recombinant Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)


    The Human Multipotent Mesenchymal Stromal Cell Marker Antibody Panel is designed for the characterization of cultured or isolated human multipotent mesenchymal stromal cells. The panel contains a group of antibodies for the positive (CD105, CD29, CD44, CD90) and negative (CD45) selection of this lineage. Multipotent mesenchymal stromal cells (MSCs) represent a heterogeneous subset of stromal cells. They can be bone marrow-derived or isolated from many other different adult tissues including periosteum, trabecular bone, adipose tissue, and synovium. They exhibit the potential to give rise to cells of diverse lineages including adipocytes, chondrocytes and osteocytes.

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)
    Ab6124 staining CD44 on Human Tissue sections (Skin tumor) by IHC-P. Sections were Formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid prior to blocking with 10% serum for 30 mins at 25°C. The primary antibody was diluted 1/100 in PBS and incubated with the sample overnight at 4°C. A TRITC non-Abcam secondary antibody was used.
  • Mesenchymal Stromal Cell Marker (CD44, CD45, CD90 CD29, CD105) Antibody Panel - Human (ab93758)
    Mesenchymal Stromal Cell Marker (CD44, CD45, CD90 CD29, CD105) Antibody Panel - Human (ab93758)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (8)

Publishing research using ab93758? Please let us know so that we can cite the reference in this datasheet.

ab93758 has been referenced in 8 publications.

  • Prautsch KM  et al. Ex-Vivo Stimulation of Adipose Stem Cells by Growth Factors and Fibrin-Hydrogel Assisted Delivery Strategies for Treating Nerve Gap-Injuries. Bioengineering (Basel) 7:N/A (2020). PubMed: 32380789
  • Prautsch KM  et al. Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro. Cells 9:N/A (2020). PubMed: 32839392
  • Bi H  et al. Stromal vascular fraction promotes migration of fibroblasts and angiogenesis through regulation of extracellular matrix in the skin wound healing process. Stem Cell Res Ther 10:302 (2019). PubMed: 31623669
  • Huang X  et al. Rho/Rho-associated protein kinase signaling pathway-mediated downregulation of runt-related transcription factor 2 expression promotes the differentiation of dental pulp stem cells into odontoblasts. Exp Ther Med 15:4457-4464 (2018). PubMed: 29731830
  • Lehner C  et al. The blood-tendon barrier: identification and characterisation of a novel tissue barrier in tendon blood vessels. Eur Cell Mater 31:296-311 (2016). PubMed: 27227787
  • Chen TT  et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). PubMed: 20176926
  • Chang HL  et al. Uterine leiomyomas exhibit fewer stem/progenitor cell characteristics when compared with corresponding normal myometrium. Reprod Sci 17:158-67 (2010). PubMed: 19805552
  • Hunt ME & Brown DR Mycoplasma alligatoris infection promotes CD95 (FasR) expression and apoptosis of primary cardiac fibroblasts. Clin Diagn Lab Immunol 12:1370-7 (2005). PubMed: 16339059

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

Customer inquiry regarding 5 antibodies in the Human Mesenchymal Stromal Cell Marker panel:
CD44 (ab6124)
CD45 (ab10559)
CD90 (ab23894)
CD105 (ab44967)
CD29 (ab52971)

Customer wondering which antibodies are positive mesenchymal stromal cell markers and which are negative. Also wondering what flow cytometry procedure is in terms of gating and the expected color associated with each antibody.

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Answer

Thank you for contacting Abcam.
Earlier today we discussed the Human Mesenchymal Stromal Cell Marker panel ab93758. Based on the information on the datasheet, I compiled the following information for you:
1. Positive selection (CD105, CD29, CD44, CD90)
2. Negative selection (CD45)
The stem cell panel ab93758 consists of 5 unconjugated primary antibodies. These antibodies can be used with the secondary antibodies against mouse or rabbit of your choice. You can therefore adapt the fluorochrome choice to the ability of your machine.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Dear Sir,
I want to know if this Product: Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758) could be use for the RABBIT mesenchymal stem cells characterization.
Anyway, could you tell me which appropriate antibodies could I use for Rabbit MSCs characterization?
Thanks in advance
Best Regards

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Answer

Thank you for your enquiry and your interest in our products.

ab93758 is a Human Mesenchymal Stromal Cell Marker Panel containing 50µg of CD44 mouse monoclonal, 50µg of CD45 rabbit polyclonal, 50µg of CD90 mouse monoclonal, 50µl of CD29 rabbit monoclonal and 50µl of CD105 mouse monoclonal antibodies.

The cross-reactivity and the tested applications of these individual antibodies can be found on the product datasheets and on the pages via these hyperlinks:

https://www.abcam.com/human-mesenchymal-stromal-cell-marker-panel-cd44-cd45-cd90-cd29-and-cd105-ab93758.html

Components of this Panel:

https://www.abcam.com/cd44-antibody-f10-44-2-ab6124.html

https://www.abcam.com/cd45-antibody-ab10559.html

https://www.abcam.com/cd90--thy1-antibody-af-9-ab23894.html

https://www.abcam.com/cd105-antibody-105c02-ab44967.html

https://www.abcam.com/integrin-beta-1-antibody-ep1041y-carboxyterminal-end-ab52971.html

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Hi,
a customer of ours is interested in your ab93758. She has co-coltures of stromal mesenchymal stem cellsand T lymphocytes isolated from bone.
She would like to detect only stromal mesenchymal stem, but not T lymphocytes. Can you please let me know if among the markers (CD44, CD45, CD90 CD29 and CD105) included within ab93758, there is some marker able to detect also T lymphocytes?

Thanks in advance and kind regards

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Answer

Thank you for your enquiry and interest in our products.

I can confirm that this panel contains 5 primary antibodies which recognize human mesenchymal stromal cells: ab6124, ab10559, ab23894, ab44967, ab52971. The datasheets can be found at these sites:

https://www.abcam.com/cd44-antibody-f10-44-2-ab6124.html

https://www.abcam.com/cd45-antibody-ab10559.html

https://www.abcam.com/cd90--thy1-antibody-af-9-ab23894.html

https://www.abcam.com/cd105-antibody-105c02-ab44967.html

https://www.abcam.com/integrin-beta-1-antibody-ep1041y-carboxyterminal-end-ab52971.html

There are some very informative and useful websites summarizing the different CD markers. You may wish to recommend to your customer for further consideration:

https://docs.abcam.com/pdf/immunology/cdantigen_poster.pdf

http://pathologyoutlines.com/cdmarkers.html

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

The CD44 and CD45 antibodies were short when they arrived

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Answer

Thank you for contacting Abcam earlier today.

I am sorry that the vials of CD44 and CD45 were shot when they arrived. As we discussed we will send out free of charge replacements for these two antibodies to make up for this shortage. Your new order number is *******.

Please let me know if there is anything else I can help you with.

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Question

Inquiry: Could you please tell me, if I can use your mesenchymal stem cell kit on a 15 year old BD FACScan? Yours sincerely

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Answer

Thank you for your inquiry.
The stem cell panel ab93758 consists of 5 unconjugated primary antibodies. These antibodies can be used with the secondary antibodies against mouse or rabbit of your choice. You can therefore adapt the fluorochrome choice to the ability of your machine.
The answer is therefore yes, you can use these primary antibodies on the an old FACS machine, if you have the adapted secondary antibodies in your laboratory.
I hope this answer is helpful. Please do not hesitate to contact us again, should you need any additional information.

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Question

Inquiry: could you please tell me if your mesenchymal stem cell test panel for flow cytometry would work on a very old FACScan system? Kind regards

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Answer

Thank you for your inquiry.

Our Mesenchymal Stromal Cell Marker Panels (human: ab93758, mouse: ab93759) consist of five un-conjugated primary antibodies that can be used to characterise mesenchymal stromal cells.

The antibodies are not conjugated and therefore require a secondary antibody with a fluorochrome.

You can choose these fluorochromes according to the FACScan system. Please contact the originator of the FACScan to ask which fluorochromes are suitable/possible to use.

I hope this information is helpful and wish you good luck with your research.

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