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Protease and Phosphatase Inhibitor Cocktail (ab201119)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (18)

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Overview

  • Product name

    Protease and Phosphatase Inhibitor Cocktail
  • Product overview

    To protect against both protease and phosphatase in one solution try our dual cocktail (EDTA-free also available).


    AEBSF free and mass spectroscopy compatible – water soluble.


    Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases


    Target phosphatases - serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases


    Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate, EDTA disodium salt.


    Form: Supplied as 1 vial of cocktail in water (1 ml) and 1 vial of EDTA disodium salt (0.5 mM in 1 ml of water)


    Preparation: The recommended starting dilution is 1:9


     


    Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K283 EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail. K283-1 is the same size as the 1 kit size of ab201119.

    Individual components and concentration:

    Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
    Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
    E-64: cysteine protease inhibitor (1.5 mM)
    Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
    β-Glycerophosphate: serine/threonine phosphatase inhibitor (10 mM)
    Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (50 mM)
    Sodium orthovanadate: protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
    Sodium pyrophosphate decahydrate: serine/threonine phosphatase inhibitor (10 mM)
    EDTA disodium salt: metalloprotease inhibitor (0.5 mM)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 kit
    EDTA Solution (10X) Clear 1 x 1ml
    Protease and Phosphatase Inhibitor Cocktail (10X) Red 1 x 1ml

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (18)

Publishing research using ab201119? Please let us know so that we can cite the reference in this datasheet.

ab201119 has been referenced in 18 publications.

  • Wen X  et al. CircRNA-011235 Counteracts The Deleterious Effect of Irradiation Treatment on Bone Mesenchymal Stem Cells by Regulating The miR-741-3p/CDK6 Pathway. Cell J 24:15-21 (2022). PubMed: 35182060
  • Jayaraman A  et al. TNF-mediated neuroinflammation is linked to neuronal necroptosis in Alzheimer's disease hippocampus. Acta Neuropathol Commun 9:159 (2021). PubMed: 34625123
  • Wang Z  et al. Integrated analysis identifies low microRNA-215 expression as associated with a poor prognosis of patients with colorectal cancer through the IKß-a signaling pathway. Transl Cancer Res 9:5233-5244 (2020). PubMed: 35117890
  • Attoff K  et al. Acrylamide alters CREB and retinoic acid signalling pathways during differentiation of the human neuroblastoma SH-SY5Y cell line. Sci Rep 10:16714 (2020). PubMed: 33028897
  • Sears JC & Broadie K FMRP-PKA Activity Negative Feedback Regulates RNA Binding-Dependent Fibrillation in Brain Learning and Memory Circuitry. Cell Rep 33:108266 (2020). PubMed: 33053340
View all Publications for this product

Customer reviews and Q&As

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Question

A customer asks for more details about using Protease and Phosphatase Inhibitor Cocktail (ab201119). Especially he would like to learn that how he uses EDTA solution.

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Abcam community

Verified customer

Asked on Dec 29 2016

Answer

All the inhibitors in the ab201119 Protease and Phosphatase Inhibitor Cocktail kit including EDTA are present in 10X concentration. The final concentration of the inhibitors in the lysate should be 1x.  EDTA solution is added to the samples in such a way that the final concentration is 1X.

Read More

Abcam Scientific Support

Answered on Dec 29 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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