Product nameProtease and Phosphatase Inhibitor Cocktail (EDTA-free)
To protect against both protease and phosphatase in one solution try our dual cocktail (also available with EDTA).
AEBSF free and mass spectroscopy compatible – water soluble.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases
Target phosphatases - Serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases
Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate
Form: Supplied in water (1 ml)
Preparation: Recommended dilution is 1:9. They are provided in a 10x formulation and a 1X solution is a good starting point. The working concentration should be optimized for each individual application.
Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.
This product is manufactured by BioVision, an Abcam company and was previously called K284 Universal Protease and Phosphatase Inhibitor Cocktail, EDTA-Free, EZBlock™. K284-1 is the same size as the 1 ml size of ab201120.
Individual components and concentrations:
Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
β-Glycerophosphate: serine/threonine phosphatase inhibitor (10 mM)
Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (50 mM)
Sodium orthovanadate: protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
Sodium pyrophosphate decahydrate: serine/threonine phosphatase inhibitor (10 mM)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 ml Protease and Phosphatase Inhibitor Cocktail (EDTA-free) 1 x 1ml
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab201120 has been referenced in 3 publications.
- Hu TY et al. Dysiarenone from Marine Sponge Dysidea arenaria Attenuates ROS and Inflammation via Inhibition of 5-LOX/NF-?B/MAPKs and Upregulation of Nrf-2/OH-1 in RAW 264.7 Macrophages. J Inflamm Res 14:587-597 (2021). PubMed: 33664584
- Hu TY et al. Anti-inflammation action of xanthones from Swertia chirayita by regulating COX-2/NF-?B/MAPKs/Akt signaling pathways in RAW 264.7 macrophage cells. Phytomedicine 55:214-221 (2019). PubMed: 30668431
- Liao S et al. Tanshinol borneol ester, a novel synthetic small molecule angiogenesis stimulator inspired by botanical formulations for angina pectoris. Br J Pharmacol 176:3143-3160 (2019). PubMed: 31116880