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  1. Link

    products/panels/protease-inhibitor-cocktail-ab65621.pdf

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Cell Biology Proteolysis / Ubiquitin Protease inhibitors Other protease inhibitors
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Protease Inhibitor Cocktail (ab65621)

  • Datasheet
  • SDS
Submit a review Q&A (14)References (25)

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Overview

  • Product name

    Protease Inhibitor Cocktail
  • Product overview

    Abcam's Protease Inhibitor Cocktail contains a mixture of water-soluble protease inhibitors with broad specificity for various proteases, and has been optimized to generate the maximal protection to mammalian tissue and cell extracts.


    Form:


    Supplied as a lyophilized powder.


    One vial can make 125 ml of cocktail solution.


    Preparation:


    Add 250 μl DMSO to the vial, pipette several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).



    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K271 Protease Inhibitor Cocktail. K271-500 is the same size as the 1 kit size of ab65621.

    Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in the extract. The individual components of the protease inhibitor cocktail have specific inhibitory properties. A description of each inhibitor is given below:

    Aprotinin: Inhibits serine proteases, such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, and kallikrein.

    Leupeptin: Inhibits both serine and cysteine proteases, such as calpain, trypsin, papain, and cathepsin B.

    Pepstain A: Inhibits acid proteases, such as pepsin, rennin, cathepsin D, chymosin, and protease B.

    PMSF: Inhibits serine proteases, such as trypsin and chymotrypsin. Also inhibits cysteine proteases and mammalian acetylcholinesterase.

     

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 kit
    Protease Inhibitor Cocktail 1 vial
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Other protease inhibitors
  • Relevance

    Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in an extract. A protease inhibitor cocktail contains a mixture of protease inhibitors with broad specificity for various proteases.
  • Alternative names

    • Aprotinin
    • Leupeptin
    • Pepstatin
    • PMSF

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (25)

Publishing research using ab65621? Please let us know so that we can cite the reference in this datasheet.

ab65621 has been referenced in 25 publications.

  • Smith P  et al. Characterization of ultrasound-mediated delivery of trastuzumab to normal and pathologic spinal cord tissue. Sci Rep 11:4412 (2021). PubMed: 33627726
  • Shatoor AS  et al. Short-term administration of C. aronia stimulates insulin signaling, suppresses fatty acids metabolism, and increases glucose uptake and utilization in the hearts of healthy rats. Saudi J Biol Sci 28:1966-1977 (2021). PubMed: 33732083
  • Agharahimi M  et al. Cocaine potentiates an inflammatory response in C6 astroglia-like cells. Biomed Rep 14:45 (2021). PubMed: 33786174
  • Qi L  et al. G-rich sequence factor 1 serves as a prognostic biomarker in septic patients. Ann Transl Med 9:691 (2021). PubMed: 33987389
  • McMahon D  et al. Investigating the effects of dexamethasone on blood-brain barrier permeability and inflammatory response following focused ultrasound and microbubble exposure. Theranostics 10:1604-1618 (2020). PubMed: 32042325
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 14 Abreviews or Q&A

Question

We have just buyed "Protease Inhibitor Cocktail (ab65621)". It is in powder form. It is written "one vial can make 125 ml of cocktail solution" on  its datasheet. Which buffer must it be used to reconstitute? Or is only "distile water" suitable?

Read More

Abcam community

Verified customer

Asked on Dec 14 2011

Answer

Thank you for contacting us.  To reconstitute this product, add 250-µl DMSO to the vial and pipet several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).  I have added these instructions to our datasheet.  I hope this helps, please let me know if you need any additional information. 

Read More

Abcam Scientific Support

Answered on Dec 14 2011

Question

Inquiry: Can it be used for saliva sample? do you have any information?

Read More

Abcam community

Verified customer

Asked on Dec 08 2014

Answer

I can confirm that ab65621 Protease Inhibitor Cocktail can be used on saliva samples.

Read More

Sam Washer

Abcam Scientific Support

Answered on Dec 08 2014

Question


Inquiry: I want to ask for how long can I store the protease inhibitor cocktail ab65621 at -20 C after reconstitution with DMSO?

Read More

Abcam community

Verified customer

Asked on Sep 24 2013

Answer

The protease inhibitor cocktail can be stored in small aliquots at -20C after reconstitution in DMSO for 1 year. We recommend to avoid freezing and thawing during this time.

Read More

Abcam Scientific Support

Answered on Sep 24 2013

Question

Thank you for your email. After we reconstitute it, do we store it a -20 degrees celcius or 4 degrees celcius?

Read More

Abcam community

Verified customer

Asked on Jan 23 2013

Answer


After reconstituting in DMSO, the cocktail should be stored at -20C.

Read More

Abcam Scientific Support

Answered on Jan 23 2013

Question

I recently purchased the protease inhibitor cocktail but there are no directions for use. We will be using it in serum to inhibit renin from converting angiotensin 1 to angiotensin 2. Can you please provide directions for use in serum and/or plasma?

Read More

Abcam community

Verified customer

Asked on Jan 22 2013

Answer


The laboratory said you can reconstitute ab65621 in 250 µl of DMSO. This makes it 250 µl of 500X inhibitor cocktail. Which means that for every 500 µl of your sample can add 1 µl of the inhibitor. These reconstitution instructions are applicable for serum, plasma, and lysate samples.

Read More

Abcam Scientific Support

Answered on Jan 22 2013

Question

Please let us have best alternate products for the above at your earliest.
1. Fluoromount Mounting medium F4680-25ML

2. Poly L Lysine cat # P8920

100G Gelbes blutlaugensalz

rotes blutlaugensalz 702587 250G

5. Hybond ECL (30 cm × 3m) 0.45um Nitrocellulose Membrane cat RPN303D

6. Amersham Hyperfilm ECL (18 x 24 cm) 50 sheets Cat # 28-9068-36

7. Amersham ECL Plus Western Blotting Detection System (18 x24 cm) 50 sheets

8. Pepstatin Cat # 20037 5MG

9. Aprotinin 11388 10 MG

10. Leupeptin 18413 5 MG

11. SAP 78390 1000 UN

12. Anti-Myc Tag, cat # clone 4A6 200

13. protein purification kit Ni-NTA column Cat.No.31014 (50 columns)

14. protein purification kit Ni-NTA slurry Cat. No.30250

15. NEB chitin-intein Tag cat # E6901S
Please update at an early time.
Sincere regards,

Read More

Abcam community

Verified customer

Asked on Sep 25 2012

Answer

Thank you for contacting us.


The alternatives are as follows

1. ab103746; https://www.abcam.com/BrightMount-25ml-Aqueous-Mounting-Medium-for-Fluorescent-Staining-ab103746.html

or ab128982; https://www.abcam.com/Aqueous-Mounting-Medium-ab128982.html

2. We do not sell Poly L Lysine




5. ab133412; https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
6. We have similar product;https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
7. Similar product;https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
8. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

9. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

10. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

11. We do not sell SAP

12. We don have clone 4A6 antibody. We however have ab32; https://www.abcam.com/c-Myc-antibody-9E10-ChIP-Grade-ab32.htmlwhich is better than clone 4A6.

13. We do not sell protein purification kit Ni-NTA column Cat.No.31014 (50 columns)

14. We do not sell protein purification kit protein purification kit Ni-NTA slurry Cat. No.30250

15. We do not sell NEB chitin-intein Tag cat # E6901S

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Sep 25 2012

Question

Hi, Thanks for your reply along with your MSDS's. Since i need CANADIAN MSDS, can i consider the MSDS you have sent, as applicable for CANADIAN Standards and regulations?

Read More

Abcam community

Verified customer

Asked on Aug 28 2012

Answer

Thank you for contacting us. To the best of our knowledge the SDS is compliant with Canadian legislation as long as it has the statement This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all the information required by the CPR. in section 15. I have attached the updated SDS with this statement for you. I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Read More

Abcam Scientific Support

Answered on Aug 28 2012

Question

Hi Sir/Madam, I am requesting an MSDS (Material Safety Data Sheet) for the below mentioned products which is used by our Client 1. Proteasa Inhibitor Cocktail Kindly send us Latest dated Canada English MSDS to below mentioned Email id. Or In the website we found latest dated USA MSDS can we consider the MSDS which we found in the website is applicable for Canadian Standards and Regulations. Request Reference Number:

Read More

Abcam community

Verified customer

Asked on Aug 24 2012

Answer

Thank you for contacting us.

Could you please provide a catalog number for this product? I did find that we do carry a Protease Inhibitors and have attached the SDS from our datasheet for that product (ab65621) however if this is not the correct product please let me know and I will locate the appropriate SDS.

This link will direct you to the datasheet for ab6562, an online copy of the SDS may be found there as well:https://www.abcam.com/protease-inhibitor-cocktail-ab65621.html


I look forward to hearing from you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Aug 24 2012

Question









I have an enquiry regarding a product that we ordered from you, protease cocktail inhibitors, and catalogue number ab65621. It is related to the Method of preparation? As it just said it is enough for 500 tests without mentioning weight and dilution factors!!!!! Please let me know if you have any useful ideas.

Read More

Abcam community

Verified customer

Asked on Aug 06 2012

Answer

This product is provided as a lyophilized powder. You have to reconstitute it in 250 µl of DMSO. This makes it 250 µl of 500X inhibitor cocktail. Which means that for every 499 or 500 µl of the lysate you can add 1 µl of the inhibitor. This means that the total amount of this inhibitor i.e., 250 µl is enough for 500 x 250 µl of the lysate, which is 125 ml of lysate.

Therefore 500 is the dilution factor, I am not sure what the weight means.

Here are some helpful references:


K271 :: Protease Inhibitor Cocktail

- Beaupre, D.M., et al. (2004) Mol. Cancer Ther. 3:179-186.

- Dabkowski, E. et al. Mitochondrial Dysfunction in the Type 2 Diabetic Heart Is Associated with Alterations in Spatially Distinct Mitochondrial Proteosomes.Am. J. Physiol. Heart Circ. Physiol.,2010;299: H529-H540.

- Janckila, A. J., et al. (2005) J. Leukoc. Biol. 77: 209-218.

- Lange S et al (2009) Cardiovasc Res; 81: 159-168.

- Mori Y et al. (2007) J. Virol. 81: 8477-8487.

- Narumi Hashikawa-Hobara et al., Histamine 3 Receptor Activation Reduces the Expression of Neuronal Angiotensin II Type 1 Receptors in the Heart,http://jpet.aspetjournals.org/content/340/1/185.abstract?maxtoshow=&HITS=10&hits=60&RESULTFORMAT=1&andorexacttitle=and&andorexacttitleabs=and&fulltext=BioVision&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=match&fdate=1/1/2012&resourcetype=HWCIT

- Ryu M-S et al (2008) J. Nutr.; 138: 2076 - 2083.

- Yun, H. et al. Physical Interaction of Jab1 with Human Serotonin 6 G-protein-coupled Receptor and Their Possible Roles in Cell Survival. J. Biol. Chem. 2010 285: 10016-10029.

Read More

Abcam Scientific Support

Answered on Aug 06 2012

Question

Hola .
Tenemos duda en que líquido se diluye este producto:
https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html
Cada vial viene liofilizado, se agregan 250ul de DMSO para dar una solución de 500X, pero para llevar el vial a 125ml a 1X, se agrega agua? Destilada, desionizada, grado biología molecular?
Saludos Cordiales.

Read More

Abcam community

Verified customer

Asked on Apr 25 2012

Answer

Gracias por contactarnos.

Para diluir el inhibidor enzimático se puede usar indistintamente agua destilada, desionizada o grado biología molecular. Siempre es más recomendable usar agua Milli Q, pero no es indispensable.

Espero haber ayudado. En caso contrario, no dudéis en volver a contactarnos.

Read More

Abcam Scientific Support

Answered on Apr 25 2012

1-10 of 14 Abreviews or Q&A

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