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Protease Inhibitor Cocktail (ab65621)

Submit a review Q&A (14)References (25)

Overview

  • Product name

    Protease Inhibitor Cocktail

  • Product overview

    Abcam's Protease Inhibitor Cocktail contains a mixture of water-soluble protease inhibitors with broad specificity for various proteases, and has been optimized to generate the maximal protection to mammalian tissue and cell extracts.


    Form:


    Supplied as a lyophilized powder.


    One vial can make 125 ml of cocktail solution.


    Preparation:


    Add 250 μl DMSO to the vial, pipette several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).



    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K271 Protease Inhibitor Cocktail. K271-500 is the same size as the 1 kit size of ab65621.

    Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in the extract. The individual components of the protease inhibitor cocktail have specific inhibitory properties. A description of each inhibitor is given below:

    Aprotinin: Inhibits serine proteases, such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, and kallikrein.

    Leupeptin: Inhibits both serine and cysteine proteases, such as calpain, trypsin, papain, and cathepsin B.

    Pepstain A: Inhibits acid proteases, such as pepsin, rennin, cathepsin D, chymosin, and protease B.

    PMSF: Inhibits serine proteases, such as trypsin and chymotrypsin. Also inhibits cysteine proteases and mammalian acetylcholinesterase.

     

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 kit
    Protease Inhibitor Cocktail 1 vial

  • Research areas

  • Relevance

    Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in an extract. A protease inhibitor cocktail contains a mixture of protease inhibitors with broad specificity for various proteases.

  • Alternative names

    • Aprotinin
    • Leupeptin
    • Pepstatin
    • PMSF

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (25)

Publishing research using ab65621? Please let us know so that we can cite the reference in this datasheet.

ab65621 has been referenced in 25 publications.

  • Smith P  et al. Characterization of ultrasound-mediated delivery of trastuzumab to normal and pathologic spinal cord tissue. Sci Rep 11:4412 (2021). PubMed: 33627726
  • Shatoor AS  et al. Short-term administration of C. aronia stimulates insulin signaling, suppresses fatty acids metabolism, and increases glucose uptake and utilization in the hearts of healthy rats. Saudi J Biol Sci 28:1966-1977 (2021). PubMed: 33732083
  • Agharahimi M  et al. Cocaine potentiates an inflammatory response in C6 astroglia-like cells. Biomed Rep 14:45 (2021). PubMed: 33786174
  • Qi L  et al. G-rich sequence factor 1 serves as a prognostic biomarker in septic patients. Ann Transl Med 9:691 (2021). PubMed: 33987389
  • McMahon D  et al. Investigating the effects of dexamethasone on blood-brain barrier permeability and inflammatory response following focused ultrasound and microbubble exposure. Theranostics 10:1604-1618 (2020). PubMed: 32042325
View all Publications for this product

Customer reviews and Q&As

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11-14 of 14 Abreviews or Q&A

Question

Dear All,



Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



Early response requested.



Sincere regards,

Read More
Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam1012-ChIP-Grade-ab1012.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam1012-ChIP-Grade-ab1012.html).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam12209-ChIP-Grade-ab12209.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam12209-ChIP-Grade-ab12209.html).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/Histone-H3-tri-methyl-K27-antibody-mAbcam-6002-ChIP-Grade-ab6002.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K27-antibody-mAbcam-6002-ChIP-Grade-ab6002.html).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/DDDDK-tag-antibody-Equivalent-to-FLAG-antibodies-from-Sigma-ab124462.html (or use the following: https://www.abcam.com/DDDDK-tag-antibody-Equivalent-to-FLAG-antibodies-from-Sigma-ab124462.html).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/Goat-Rabbit-IgG-HL-Cy3--preadsorbed-ab6939.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-Cy3--preadsorbed-ab6939.html).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-preadsorbed-ab96879.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-preadsorbed-ab96879.html).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-ab97265.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-ab97265.html).

ab7481 (Goat Serum)

https://www.abcam.com/Normal-Goat-Serum-ab7481.html (or use the following: https://www.abcam.com/Normal-Goat-Serum-ab7481.html).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/Normal-Rabbit-Serum-Sterile-ab7487.html (or use the following: https://www.abcam.com/Normal-Rabbit-Serum-Sterile-ab7487.html).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/Herring-sperm-ssDNA-ab46666.html (or use the following: https://www.abcam.com/Herring-sperm-ssDNA-ab46666.html).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/Natural-cow-Ribonuclease-A-protein-ab52579.html (or use the following: https://www.abcam.com/Natural-cow-Ribonuclease-A-protein-ab52579.html).

ab73430 (DNase I protein (Active))

https://www.abcam.com/Recombinant-human-DNase-I-protein-Active-ab73430.html (or use the following: https://www.abcam.com/Recombinant-human-DNase-I-protein-Active-ab73430.html).

ab64220 (Proteinase K)

https://www.abcam.com/Proteinase-K-Antigen-Retrieval-Solution-ab64220.html (or use the following: https://www.abcam.com/Proteinase-K-Antigen-Retrieval-Solution-ab64220.html).

ab65621 (Protease Inhibitor Cocktail)

https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html (or use the following: https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/Prestained-Protein-Ladder-Broad-molecular-weight-10-245-kDa-ab116028.html (or use the following: https://www.abcam.com/Prestained-Protein-Ladder-Broad-molecular-weight-10-245-kDa-ab116028.html).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Question

Will this protease inhibitor cocktail inhibit the cathepsin family?

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Answer

Thank you for your call today and for your patience while I have worked on your enquiry.

I have confirmed that the cathepsins are serine, cysteine, or aspartyl (acidic) proteases, all of which are blocked by this cocktail.

I hope this information will be useful in deciding if this product will work for you, but please let me know if you have any further questions or if there is anything else that we can do for you.

Read More

Question

Mitochondria Isolation Kits for Cultured Cells (ab110170 and ab110171) and Protease Inhibitor Cocktail (ab65621) were bought together. Could you please inform how much Protease Inhibitor Cocktail (ab65621) must be added to Reagent C in the kits?

Read More
Answer

Thank you for contacting us.

I can confirm that 1 µl of Protease Inhibitor Cocktail ab65621 must be added to Reagent C (both ab110170 and ab110171):

As stated on the datasheet of ab65621, you need to add 250 µl DMSO to the vial and pipet several times to dissolve all powder. This makes a 500-fold concentrated inhibitor cocktail stock solution.

According to the protocols of the Mitochondria Isolation Kits, the pellet is to be resuspended into 500 µl of Reagent C supplemented with Protease Inhibitors, i.e. with 1 µl of the Protease Inhibitor Cocktail stock solution.

We would recommend to add the Protease Inhibitor Cocktail always freshly to Reagent C for each sample preparation to ensure fully active inhibitors.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Thank you for your swift reply. I think the customer can't have DMSO in own lab. Could you please recommend another solvent that can be more common in labs; like acetone (a polar aprotic solvent like DMSO), H2O or ROH (polar protic solvents)? By the way, I have just realized that It is written "The Protease Inhibitor Cocktail contains a mixture of water-soluble protease inhibitors with broad specificity for various proteases" on the datasheet. So, I am planning to recommend a water reconstitution to our customer If you wouldn't recommend another solvent.

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Answer

I apologize for the delay in my reply. The lab has confirmed that the protease inhibitor cocktail can also be solubilized in water before diluting them in media or buffers of your choice. I hope this helps, please let me know if you need any additional information.

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11-14 of 14 Abreviews or Q&A

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