Product nameRegulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human
Species reactivityReacts with: Human
Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human ab254021 contains multiple trial-sized versions of anti-human antibody clones against CD4, FOXP3, CTLA4, CCR6, HLA-DR, specifically selected for high performance in various applications. This panel contains 5 recombinant rabbit monoclonal antibodies against human CD4, FOXP3, CTLA4, CCR6 and 1 mouse monoclonal antibody against HLA-DR. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
- Rabbit monoclonal [EPR6855] to CD4 (20 µL) ab133616
- Rabbit monoclonal [EPR22102-37] to FOXP3 (20 µL) ab215206
- Rabbit monoclonal [CAL49] to CTLA4 (20 µL) ab237712
- Rabbit monoclonal [EPR22259] to CCR6 (20 µL) ab227036
- Mouse monoclonal [TAL 1B5] to HLA-DR (20 µg) ab20181
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit ab227036 - Anti-CCR6 antibody [EPR22259] 2 x 10µl ab133616 - Anti-CD4 antibody [EPR6855] 2 x 10µl ab237712 - Anti-CTLA4 antibody [CAL49] 2 x 10µl ab215206 - Anti-FOXP3 antibody [EPR22102-37] 2 x 10µl ab20181 - Anti-HLA-DR antibody [TAL 1B5] 2 x 10µg
Cellular localizationCD4: Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. HLA-DR: Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. CTLA4: Cell membrane. Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation and. CCR6: Cell membrane. FOXP3: Nucleus.
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
- Anti-CD4 antibody [EPR6855] - BSA and Azide free (ab181724)
- Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (ab196147)
- Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (ab196372)
- Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)
- Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
- Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
- Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)
Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling FOXP3 with ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in human thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
Anti-CD4 antibody [EPR6855] (ab133616)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD4 with ab133616 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.
IHC image of HLA-DR [TAL 1B5] staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20181, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of paraffin-embedded human Hodgkin’s lymphoma tissue labeling CCR6 with ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human Hodgkin’s lymphoma (PMID: 12514792) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD4 with purified ab133616 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254021 has not yet been referenced specifically in any publications.