Anti-4-1BBL antibody [014] - BSA and Azide free (ab277140)
Key features and details
- Rabbit monoclonal [014] to 4-1BBL - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-4-1BBL antibody [014] - BSA and Azide free
See all 4-1BBL primary antibodies -
Description
Rabbit monoclonal [014] to 4-1BBL - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse 4-1BBL aa 104-309. Genbank: NP_033430.1
Database link: P41274 -
Positive control
- Flow cyt: RAW264.7 cells ICC: RAW264.7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS
0.2 µm filtered solution -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
014 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
1/50 - 1/1000.
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Flow Cyt |
1/25 - 1/100.
|
Notes |
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ICC
1/50 - 1/1000. |
Flow Cyt
1/25 - 1/100. |
Target
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Function
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. -
Tissue specificity
Expressed in brain, placenta, lung, skeletal muscle and kidney. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 21950 Mouse
- SwissProt: P41274 Mouse
- Unigene: 41171 Mouse
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Alternative names
- 4 1BB L antibody
- 4 1BB ligand antibody
- 4 1BBL antibody
see all
Images
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Flow cytometric analysis of 4-1BBL expression on Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. Cells were stained with ab277140 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also staibed with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Confocal immunofluorescence analysis of 4-1BBL in RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277140 at 1/100 dilution at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab277140 has not yet been referenced specifically in any publications.