Recombinant Anti-4-1BBL antibody [EPR1172Y] (ab68185)
![Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)](https://www.abcam.com/ps/products/68/ab68185/Images/ab68185-253952-anti-4-1bbl-antibody-epr1172y-western-blot.jpg "Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1172Y] to 4-1BBL
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-4-1BBL antibody [EPR1172Y]
See all 4-1BBL primary antibodies -
Description
Rabbit monoclonal [EPR1172Y] to 4-1BBL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human 4-1BBL (C terminal). The exact sequence is proprietary.
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Positive control
- WB: 293T and Raji cell lysates. IP: HEK293 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1172Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
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| IP |
1/40 - 1/50.
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| Notes |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa). |
|
IP
1/40 - 1/50. |
Target
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Function
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. -
Tissue specificity
Expressed in brain, placenta, lung, skeletal muscle and kidney. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8744 Human
- Omim: 606182 Human
- SwissProt: P41273 Human
- Unigene: 1524 Human
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Alternative names
- 4 1BB L antibody
- 4 1BB ligand antibody
- 4 1BBL antibody
see all
Images
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Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + Raji whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25,32 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)](https://www.abcam.com/ps/products/68/ab68185/Images/ab68185-253951-anti-4-1bbl-antibody-epr1172y-western-blot.jpg)
Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + HEK293 whole cell lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25-32 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (ab68185)](https://www.abcam.com/ps/products/68/ab68185/Images/ab68185-253854-anti-4-1bbl-antibody-epr1172y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (ab68185)ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.
Lane 1 (input): HEK293 whole cell lysate (10µg)
Lane 2 (+): ab68185 + HEK293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)](https://www.abcam.com/ps/products/68/ab68185/Images/ab68185-82382-anti-4-1bbl-antibody-epr1172y-western-blot.jpg)
Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/2000 dilution (unpurified) + 293T cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
![Anti-4-1BBL antibody [EPR1172Y] (ab68185)](https://www.abcam.com/ps/products/68/ab68185/Images/ab68185-5-benefits-of-recombinant-antibodies.png)
Anti-4-1BBL antibody [EPR1172Y] (ab68185)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab68185 has been referenced in 1 publication.
- Fu X et al. Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat. Respir Res 22:52 (2021). PubMed: 33579290