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    products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185.pdf

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RecombinantRabMAb

Recombinant Anti-4-1BBL antibody [EPR1172Y] (ab68185)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
  • Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
  • Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
  • Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
  • Anti-4-1BBL antibody [EPR1172Y] (ab68185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1172Y] to 4-1BBL
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-4-1BBL antibody [EPR1172Y]
    See all 4-1BBL primary antibodies
  • Description

    Rabbit monoclonal [EPR1172Y] to 4-1BBL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human 4-1BBL (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: 293T and Raji cell lysates. IP: HEK293 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1172Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Cancer
    • Growth factors
    • TNF

Associated products

  • Alternative Versions

    • HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523)
    • Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • HEK-293 whole cell lysate (ab7902)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
IP
1/40 - 1/50.
Notes
WB
1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
IP
1/40 - 1/50.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
  • Tissue specificity

    Expressed in brain, placenta, lung, skeletal muscle and kidney.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P41273 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8744 Human
    • Omim: 606182 Human
    • SwissProt: P41273 Human
    • Unigene: 1524 Human
    • Alternative names

      • 4 1BB L antibody
      • 4 1BB ligand antibody
      • 4 1BBL antibody
      • 4-1BB ligand antibody
      • 4-1BBL antibody
      • Cd137l antibody
      • Cd157l antibody
      • Homolog of mouse 4 1BB L antibody
      • Homolog of mouse 4 1BBL antibody
      • ILA ligand (TNF related) antibody
      • Ly63l antibody
      • Receptor 4 1BB ligand antibody
      • TNF superfamily member 9 antibody
      • TNFL9_HUMAN antibody
      • Tnfsf9 antibody
      • TNLG5A antibody
      • Tumor necrosis factor (ligand) superfamily member 9 antibody
      • Tumor necrosis factor ligand 5A antibody
      • Tumor necrosis factor ligand superfamily member 9 antibody
      • Tumor necrosis factor superfamily member 9 antibody
      see all

    Images

    • Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
      Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
      Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + Raji whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 27 kDa
      Observed band size: 25,32 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
      Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
      Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + HEK293 whole cell lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size: 27 kDa
      Observed band size: 25-32 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
      Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (ab68185)

      ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.

      Lane 1 (input): HEK293 whole cell lysate (10µg)

      Lane 2 (+): ab68185 + HEK293 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
      Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185)
      Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/2000 dilution (unpurified) + 293T cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 27 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?

    • Anti-4-1BBL antibody [EPR1172Y] (ab68185)
      Anti-4-1BBL antibody [EPR1172Y] (ab68185)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab68185? Please let us know so that we can cite the reference in this datasheet.

    ab68185 has been referenced in 1 publication.

    • Fu X  et al. Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat. Respir Res 22:52 (2021). PubMed: 33579290

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    WHAT TISSUE SHOULD BE TAKEN AS POSITIVE CONTROL FOR CD137L. WHAT DILUTION IS SUGGESTED?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 12 2013

    Answer


    For western blots and immunocytochemistry (ICC), we recommend HEK293T cell lysate for a positive control, and an antibody dilution of 1/500 -1/2000 for western blotting and 1/100 - 1/250 for ICC. We have the lysate as ab95494.

    Click here (or use the following: https://www.abcam.com/293T-whole-cell-lysate-ab95494.html).

    For immunhistochemistry (IHC), there are a number of choices for positive controls, as it is expressed in brain, placenta, lung, skeletal muscle and kidney. Dilution for IHC should be 1/100 - 1/250.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Aug 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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