Recombinant Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-408232-anti-4-1bbl-antibody-epr1172y-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1172Y] to 4-1BBL - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free
See all 4-1BBL primary antibodies -
Description
Rabbit monoclonal [EPR1172Y] to 4-1BBL - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247373 is the carrier-free version of ab68185.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1172Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
|
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa). |
|
IP
Use at an assay dependent concentration. |
Target
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Function
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. -
Tissue specificity
Expressed in brain, placenta, lung, skeletal muscle and kidney. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8744 Human
- Omim: 606182 Human
- SwissProt: P41273 Human
- Unigene: 1524 Human
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Alternative names
- 4 1BB L antibody
- 4 1BB ligand antibody
- 4 1BBL antibody
see all
Images
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Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + Raji whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25,32 kDa why is the actual band size different from the predicted?This data was developed using ab68185, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-405505-anti-4-1bbl-antibody-epr1172y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + HEK293 whole cell lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25-32 kDa why is the actual band size different from the predicted?This data was developed using ab68185, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-403555-anti-4-1bbl-antibody-epr1172y-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)This data was developed using ab68185, the same antibody clone in a different buffer formulation.
ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.
Lane 1 (input): HEK293 whole cell lysate (10µg)
Lane 2 (+): ab68185 + HEK293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-397912-anti-4-1bbl-antibody-epr1172y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/2000 dilution (unpurified) + 293T cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?This data was developed using ab68185, the same antibody clone in a different buffer formulation.
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![Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)](https://www.abcam.com/ps/products/247/ab247373/Images/ab247373-5-benefits-of-recombinant-antibodies.png)
Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247373 has not yet been referenced specifically in any publications.
