Recombinant Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)]
See all 5 Lipoxygenase/5-LO primary antibodies -
Description
Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: DogDoes not react with: Mouse
-
Immunogen
Synthetic peptide within Human 5 Lipoxygenase/5-LO aa 100-200. The exact sequence is proprietary.
Database link: P09917 -
Positive control
- Fetal thymus, K562 and HUVEC lysates. Human prostate and Human tonsil tissue. HUVEC cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP6072(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/2000. Predicted molecular weight: 78 kDa.
|
|
IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
|
ICC/IF |
1/100.
|
Notes |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Predicted molecular weight: 78 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
ICC/IF
1/100. |
Target
-
Function
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. -
Pathway
Lipid metabolism; leukotriene A4 biosynthesis. -
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
Post-translational
modificationsSerine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. -
Cellular localization
Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association. - Information by UniProt
-
Database links
- Entrez Gene: 477753 Dog
- Entrez Gene: 240 Human
- Entrez Gene: 25290 Rat
- Omim: 152390 Human
- SwissProt: P09917 Human
- SwissProt: P12527 Rat
- Unigene: 89499 Human
- Unigene: 9662 Rat
-
Alternative names
- 5 Lipoxygenase antibody
- 5 LO antibody
- 5 LOX antibody
see all
Images
-
All lanes : Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution
Lane 1 : Rat heart lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 78 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
ab169755 staining 5 Lipoxygenase/5-LO in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
ab169755 staining 5 Lipoxygenase/5-LO in HUVEC (human umbilical vein/vascular endothelium) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/130. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (black).
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (blue).
-
Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution + K562 cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 78 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Human fetal thymus at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 78 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Immunofluorescent analysis of HUVEC cells labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.
-
All lanes : Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution (unpurified)
Lane 1 : Fetal thymus lysate
Lane 2 : K562 lysate
Lane 3 : HUVEC lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (27)
ab169755 has been referenced in 27 publications.
- Weisser H et al. Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function. Cancer Gene Ther 30:108-123 (2023). PubMed: 36114329
- Wei TT et al. Interferon-γ induces retinal pigment epithelial cell Ferroptosis by a JAK1-2/STAT1/SLC7A11 signaling pathway in Age-related Macular Degeneration. FEBS J 289:1968-1983 (2022). PubMed: 34741776
- Yang J et al. Development and validation of a novel 3-gene prognostic model for pancreatic adenocarcinoma based on ferroptosis-related genes. Cancer Cell Int 22:21 (2022). PubMed: 35033072
- Liu N et al. TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease. J Neuroinflammation 19:26 (2022). PubMed: 35093118
- Su HY et al. Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 35563304