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RecombinantRabMAb

Recombinant Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

Reviews (1) Submit a question References (27)
Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
  • Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 APC Carrier Free PE

Overview

  • Product name

    Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)]
    See all 5 Lipoxygenase/5-LO primary antibodies

  • Description

    Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: DogDoes not react with: Mouse

  • Immunogen

    Synthetic peptide within Human 5 Lipoxygenase/5-LO aa 100-200. The exact sequence is proprietary.
    Database link: P09917

  • Positive control

    • Fetal thymus, K562 and HUVEC lysates. Human prostate and Human tonsil tissue. HUVEC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/2000. Predicted molecular weight: 78 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
ICC/IF
1/100.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/2000. Predicted molecular weight: 78 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
ICC/IF
1/100.

Target

  • Function

    Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

  • Pathway

    Lipid metabolism; leukotriene A4 biosynthesis.

  • Sequence similarities

    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.

  • Post-translational
    modifications

    Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

  • Cellular localization

    Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5 Lipoxygenase antibody
      • 5 LO antibody
      • 5 LOX antibody
      • 5-lipoxygenase antibody
      • 5-LO antibody
      • 5-LOX antibody
      • 5LOX antibody
      • 5LPG antibody
      • ALOX 5 antibody
      • Alox5 antibody
      • Arachidonate 5 lipoxygenase antibody
      • Arachidonate 5-lipoxygenase antibody
      • arachidonic 5-lipoxygenase alpha-10 isoform antibody
      • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-p10 isoform antibody
      • Arachidonic acid 5 lipoxygenase antibody
      • Leukotriene A4 synthase antibody
      • LOG 5 antibody
      • LOG5 antibody
      • LOX5_HUMAN antibody
      • MGC163204 antibody
      see all

    Images

    • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      All lanes : Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution

      Lane 1 : Rat heart lysate
      Lane 2 : Rat kidney lysate
      Lane 3 : Rat spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 78 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

      ab169755 staining 5 Lipoxygenase/5-LO in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

      Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

      ab169755 staining 5 Lipoxygenase/5-LO in HUVEC (human umbilical vein/vascular endothelium) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/130. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: Rabbit monoclonal IgG (black). 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (blue). 

       

       

    • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution + K562 cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 78 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Human fetal thymus at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 78 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

      Immunofluorescent analysis of HUVEC cells labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

    • Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      All lanes : Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal thymus lysate
      Lane 2 : K562 lysate
      Lane 3 : HUVEC lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 78 kDa

    • Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
      Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)

    References (27)

    Publishing research using ab169755? Please let us know so that we can cite the reference in this datasheet.

    ab169755 has been referenced in 27 publications.

    • Weisser H  et al. Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function. Cancer Gene Ther 30:108-123 (2023). PubMed: 36114329
    • Wei TT  et al. Interferon-γ induces retinal pigment epithelial cell Ferroptosis by a JAK1-2/STAT1/SLC7A11 signaling pathway in Age-related Macular Degeneration. FEBS J 289:1968-1983 (2022). PubMed: 34741776
    • Yang J  et al. Development and validation of a novel 3-gene prognostic model for pancreatic adenocarcinoma based on ferroptosis-related genes. Cancer Cell Int 22:21 (2022). PubMed: 35033072
    • Liu N  et al. TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease. J Neuroinflammation 19:26 (2022). PubMed: 35093118
    • Su HY  et al. Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 35563304
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    ELISA abreview for Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    ELISA
    Sample
    Mouse Cell (Microphage)
    Specification
    Microphage
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Type
    Indirect
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 12 2020

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