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RecombinantRabMAb

Recombinant Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
  • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
  • Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 APC Unconjugated

Overview

  • Product name

    Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free
    See all 5 Lipoxygenase/5-LO primary antibodies

  • Description

    Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: DogDoes not react with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal thymus, K562 and HUVEC lysates. Human prostate and Human tonsil tissue. HUVEC cells.

  • General notes

    ab227991 is the carrier-free version of ab169755.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.

Target

  • Function

    Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

  • Pathway

    Lipid metabolism; leukotriene A4 biosynthesis.

  • Sequence similarities

    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.

  • Post-translational
    modifications

    Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

  • Cellular localization

    Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5 Lipoxygenase antibody
      • 5 LO antibody
      • 5 LOX antibody
      • 5-lipoxygenase antibody
      • 5-LO antibody
      • 5-LOX antibody
      • 5LOX antibody
      • 5LPG antibody
      • ALOX 5 antibody
      • Alox5 antibody
      • Arachidonate 5 lipoxygenase antibody
      • Arachidonate 5-lipoxygenase antibody
      • arachidonic 5-lipoxygenase alpha-10 isoform antibody
      • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-p10 isoform antibody
      • Arachidonic acid 5 lipoxygenase antibody
      • Leukotriene A4 synthase antibody
      • LOG 5 antibody
      • LOG5 antibody
      • LOX5_HUMAN antibody
      • MGC163204 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
      Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

      ab169755 staining 5 Lipoxygenase/5-LO in HUVEC (human umbilical vein/vascular endothelium) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/130. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: Rabbit monoclonal IgG (black). 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (blue). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
      Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

      Immunofluorescent analysis of HUVEC cells labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

      Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

      This IHC data was generated using the same anti-5 Lipoxygenase/5-LO antibody clone, EP6072(2), in a different buffer formulation (ab169755).

      ab169755 staining 5 Lipoxygenase/5-LO in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    • Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
      Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab227991? Please let us know so that we can cite the reference in this datasheet.

    ab227991 has been referenced in 1 publication.

    • Li C  et al. Qishen granules inhibit myocardial inflammation injury through regulating arachidonic acid metabolism. Sci Rep 6:36949 (2016). WB . PubMed: 27833128

    Customer reviews and Q&As

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