Anti-53BP1 antibody (ab172580)
Key features and details
- Rabbit polyclonal to 53BP1
- Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-53BP1 antibody
See all 53BP1 primary antibodies -
Description
Rabbit polyclonal to 53BP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human 53BP1 aa 1400-1700.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Constituent: Water -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 214 kDa.
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| ICC/IF |
1/1000.
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P |
1/1000.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 214 kDa. |
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ICC/IF
1/1000. |
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IP
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P
1/1000. |
Target
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Function
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
Involvement in disease
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
Sequence similarities
Contains 2 BRCT domains. -
Post-translational
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Entrez Gene: 296099 Rat
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
see all -
Alternative names
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (ab172580)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - 53BP1, Middle - DAPI, Bottom - Merge.
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Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (ab172580)Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature.
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Flow Cytometry - Anti-53BP1 antibody (ab172580)Flow Cytometry analysis of HeLa cells labelling 53BP1 with ab172580 at 1:800. Cells were fixed with 1% PFA. Cells were incuabted with the primary antibody overnight at 4°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab172580)Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling 53BP1 with ab172580 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9.
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Immunoprecipitation - Anti-53BP1 antibody (ab172580)Immunoprecipitation analysis of U2OS cells labelling 53BP1 with ab172580. Protein A beads were crosslinked to the serum.
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Western blot - Anti-53BP1 antibody (ab172580)All lanes : Anti-53BP1 antibody (ab172580)
Lane 1 : U2OS (Control si)
Lane 2 : U2OS (53BP1 si)
Lane 3 : NIH 3T3 cell lysate at 40 µg
Lane 4 : PC12 cell lysate at 40 µg
Predicted band size: 214 kDa
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab172580 has been referenced in 16 publications.
- Gai X et al. Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response. Cell Res 32:254-268 (2022). IF ; Human . PubMed: 34980897
- Gionchiglia N et al. Association of Caspase 3 Activation and H2AX ? Phosphorylation in the Aging Brain: Studies on Untreated and Irradiated Mice. Biomedicines 9:N/A (2021). IF ; Mouse . PubMed: 34572352
- Alonso-de Vega I et al. PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaks. Nucleic Acids Res N/A:N/A (2020). WB ; Human . PubMed: 32232336
- Takasugi M et al. Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties. Nat Commun 11:2376 (2020). ICC/IF ; Human . PubMed: 32398747
- Guo G et al. miR-7/SP1/TP53BP1 axis may play a pivotal role in NSCLC radiosensitivity. Oncol Rep 44:2678-2690 (2020). WB ; Human . PubMed: 33125142
