Anti-53BP1 antibody (ab36823)
Key features and details
- Rabbit polyclonal to 53BP1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-53BP1 antibody
See all 53BP1 primary antibodies -
Description
Rabbit polyclonal to 53BP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human 53BP1 aa 350-450.
Database link: Q12888 -
Positive control
- IHC-P: Human prostate cancer tissue, mouse renal cancer tissue. WB: HeLa and mouse TCMK1 cell nuclear extract. IP: HEK293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
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| IHC-P | (1) |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa). |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
Target
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Function
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
Involvement in disease
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
Sequence similarities
Contains 2 BRCT domains. -
Post-translational
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
- Unigene: 481841 Mouse
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Alternative names
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab36823)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate cancer tissue, labeling CTCF with ab36823 at a 1/5000 dilution. Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab36823)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling CTCF with ab36823 at a 1/1000 dilution. Detection: DAB.
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Western blot - Anti-53BP1 antibody (ab36823)All lanes : Anti-53BP1 antibody (ab36823) at 0.04 µg/ml
Lane 1 : HeLa cell nuclear extract at 50 µg
Lane 2 : HeLa cell nuclear extract at 5 µg
Lane 3 : TCMK1 mouse cell nuclear extract at 15 µg
Predicted band size: 214 kDa
Exposure time: 30 secondsDetection: Chemiluminescence. -
Immunoprecipitation - Anti-53BP1 antibody (ab36823)53BP1 was immunoprecipitated from 1 mg HEK293T whole cell lysate with ab36823 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab243868 at a 1/1000 dilution.
Lane 1: ab36823 IP in HEK293T whole cell lysate.
Lane 2: Contol IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (117)
ab36823 has been referenced in 117 publications.
- Weyburne E & Bosco G Cancer-associated mutations in the condensin II subunit CAPH2 cause genomic instability through telomere dysfunction and anaphase chromosome bridges. J Cell Physiol 236:3579-3598 (2021). PubMed: 33078399
- Baquero JM et al. Small molecule inhibitor of OGG1 blocks oxidative DNA damage repair at telomeres and potentiates methotrexate anticancer effects. Sci Rep 11:3490 (2021). PubMed: 33568707
- Carmell N et al. Identification and Validation of ERK5 as a DNA Damage Modulating Drug Target in Glioblastoma. Cancers (Basel) 13:N/A (2021). PubMed: 33668183
- Hu M et al. Antitumour activity of TH1579, a novel MTH1 inhibitor, against castration-resistant prostate cancer. Oncol Lett 21:62 (2021). PubMed: 33281973
- Fütterer A et al. Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels. Cell Death Dis 12:637 (2021). PubMed: 34155199