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RecombinantRabMAb

Recombinant Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

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Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Flow Cytometry (Intracellular) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Flow Cytometry (Intracellular) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
  • Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20842] to ABAT/GABA-T - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free
    See all ABAT/GABA-T primary antibodies

  • Description

    Rabbit monoclonal [EPR20842] to ABAT/GABA-T - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    ab233702 is the carrier-free version of ab216465.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab233702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

  • Tissue specificity

    Liver > pancreas > brain > kidney > heart > placenta.

  • Involvement in disease

    Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.

  • Sequence similarities

    Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • (S) 3 amino 2 methylpropionate transaminase antibody
    • (S)-3-amino-2-methylpropionate transaminase antibody
    • 4 aminobutyrate aminotransferase antibody
    • 4 aminobutyrate aminotransferase, mitochondrial antibody
    • 4-aminobutyrate aminotransferase antibody
    • ABAT antibody
    • FLJ17813 antibody
    • FLJ30272 antibody
    • GABA aminotransferase antibody
    • GABA AT antibody
    • GABA T antibody
    • GABA transaminase antibody
    • GABA transferase antibody
    • GABA-AT antibody
    • GABA-T antibody
    • GABAT antibody
    • GABT_HUMAN antibody
    • Gamma amino N butyrate transaminase antibody
    • Gamma-amino-N-butyrate transaminase antibody
    • hCG1984265 antibody
    • L AIBAT antibody
    • L-AIBAT antibody
    • LAIBAT antibody
    • mitochondrial antibody
    • NPD009 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunofluorescent analysis of 100% methanol-fixed HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling ABAT/GABA-T with ab216465 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 (green). Confocal image showing mitochondrial staining in HepG2 cell line is observed. The nuclear counter stain is DAPI (blue).

    Mitochondria are stained with ab33985 Anti-COX IV (mouse mAb) - Mitochondrial Marker followed by ab150120 AlexaFluor®594 Goat anti-Mouse secondary both at 1/1000 dilution (red).

    -ve control 1: ab216465 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab33985 Anti-COX IV (mouse mAb) - Mitochondrial Marker at 1/1000 dilution followed by ab150077 (AlexaFluor®488 Goat anti-Rabbit secondary) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunofluorescent analysis of 100% methanol-fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling ABAT/GABA-T with ab216465 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 (green). Confocal image showing mitochondrial staining in MCF7 cell line. The nuclear counter stain is DAPI (blue).

    Mitochondria are stained with ab33985 Anti-COX IV (mouse mAb) - Mitochondrial Marker followed by ab150120 AlexaFluor®594 Goat anti-Mouse secondary both at 1/1000 dilution (red).

    -ve control 1: ab216465 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab33985 Anti-COX IV (mouse mAb) - Mitochondrial Marker at 1/1000 dilution followed by ab150077 (AlexaFluor®488 Goat anti-Rabbit secondary) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    ABAT/GABA-T was immunoprecipitated from 0.35 mg MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate with ab216465 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216465 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).
    Lane 2: ab216465 IP in MCF7 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216465 in MCF7 whole cell lysate (-).


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Flow Cytometry (Intracellular) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Flow Cytometry (Intracellular) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling ABAT/GABA-T with ab216465 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465). 

     

     

  • Flow Cytometry (Intracellular) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Flow Cytometry (Intracellular) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling ABAT/GABA-T with ab216465 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465). 

     

     

  • Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling ABAT/GABA-T with ab216465 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in rat liver is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat choroid plexus tissue labeling ABAT/GABA-T with ab216465 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in rat choroid plexus (PMID:25239459, PMID: 11459221) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse choroid plexus tissue labeling ABAT/GABA-T with ab216465 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in mouse choroid plexus (PMID:25239459, PMID: 11459221) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunohistochemistry (Frozen sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling ABAT/GABA-T with ab216465 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Cytoplasmic staining in mouse cerebellum (PMID:25239459, PMID: 11459221) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ABAT/GABA-T with ab216465 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Granular cytoplasmic staining in rat liver (PMID: 25771305; PMID: 25738457) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling ABAT/GABA-T with ab216465 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Granular cytoplasmic staining in mouse testis (PMID: 25771305; PMID: 25738457) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ABAT/GABA-T with ab216465 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Granular cytoplasmic staining in human breast cancer (PMID: 25771305; PMID: 25738457) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ABAT/GABA-T with ab216465 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Granular cytoplasmic staining in human kidney (PMID: 25771305; PMID: 25738457) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216465).

  • Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)
    Anti-ABAT/GABA-T antibody [EPR20842] - BSA and Azide free (ab233702)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab233702? Please let us know so that we can cite the reference in this datasheet.

ab233702 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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