Recombinant Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
![Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)](https://www.abcam.com/ps/products/108/ab108249/Images/ab108249-310250-anti-abat-gaba-t-antibody-epr4433-western-blot.jpg "Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4433] to ABAT/GABA-T
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ABAT/GABA-T antibody [EPR4433]
See all ABAT/GABA-T primary antibodies -
Description
Rabbit monoclonal [EPR4433] to ABAT/GABA-T -
Host species
Rabbit -
Specificity
Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only. -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 cell lysate, SH-SY5Y cell lysae, Human fetal liver tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4433 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
|
| IP |
1/10 - 1/100.
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. |
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 56 kDa. |
|
IP
1/10 - 1/100. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. |
Target
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Function
Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine. -
Tissue specificity
Liver > pancreas > brain > kidney > heart > placenta. -
Involvement in disease
Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. -
Sequence similarities
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 18 Human
- Entrez Gene: 268860 Mouse
- Entrez Gene: 81632 Rat
- Omim: 137150 Human
- SwissProt: P80404 Human
- SwissProt: P61922 Mouse
- SwissProt: P50554 Rat
- Unigene: 336768 Human
see all -
Alternative names
- (S) 3 amino 2 methylpropionate transaminase antibody
- (S)-3-amino-2-methylpropionate transaminase antibody
- 4 aminobutyrate aminotransferase antibody
see all
Images
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Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)All lanes : Anti-ABAT/GABA-T antibody [EPR4433] (ab108249) at 1/10000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM /TBST
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates with 5% NFDM /TBST
Lane 3 : Mouse brain lysates with 5% NFDM /TBST
Lane 4 : Rat brain lysates with 5% NFDM /TBST
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)](https://www.abcam.com/ps/products/108/ab108249/Images/ab108249-310260-anti-abat-gaba-t-antibody-epr4433-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)](https://www.abcam.com/ps/products/108/ab108249/Images/ab108249-310278-anti-abat-gaba-t-antibody-epr4433-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)ab108249 (purified) at 1:40 dilution (2µg) immunoprecipitating ABAT/GABA-T in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab108249 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108249 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)](https://www.abcam.com/ps/products/108/ab108249/Images/ab108249-310259-anti-abat-gaba-t-antibody-epr4433-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)](https://www.abcam.com/ps/products/108/ab108249/Images/ab108249-310258-anti-abat-gaba-t-antibody-epr4433-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ABAT/GABA-T with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)](https://www.abcam.com/ps/products/108/ab108249/Images/ab108249-120756-anti-abat-gaba-t-antibody-epr4433-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)Unpurified ab108249, at 1/250 dilution, staining ABAT/GABA-T in Human liver tissue by immunohistochemistry.
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![Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)](https://www.abcam.com/ps/products/108/ab108249/Images/ab108249-120757-anti-abat-gaba-t-antibody-epr4433-western-blot.jpg)
Western blot - Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)All lanes : Anti-ABAT/GABA-T antibody [EPR4433] (ab108249) (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Human fetal liver tissue lysate
Lane 4 : rat brain tissue lysate
Lane 5 : rat kidney tissue lysate
Lane 6 : mouse brain tissue lysate
Predicted band size: 56 kDa -
![Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)](https://www.abcam.com/ps/products/108/ab108249/Images/ab108249-9-benefits-of-recombinant-antibodies.png)
Anti-ABAT/GABA-T antibody [EPR4433] (ab108249)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab108249 has been referenced in 8 publications.
- Zhang M et al. Gamma-Aminobutyrate Transaminase Protects against Lipid Overload-Triggered Cardiac Injury in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35216295
- Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
- Park MG et al. High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays. J Enzyme Inhib Med Chem 36:2016-2024 (2021). PubMed: 34514924
- Si W et al. Effect of GABA-T on Reproductive Function in Female Rats. Animals (Basel) 10:N/A (2020). PubMed: 32230949
- Puleston DJ et al. Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. Cell Metab 30:352-363.e8 (2019). PubMed: 31130465
- Chen L et al. Involvement of upregulation of miR-210 in a rat epilepsy model. Neuropsychiatr Dis Treat 12:1731-7 (2016). WB . PubMed: 27471387
- De Ita-Pérez D et al. Synchronization by food access modifies the daily variations in expression and activity of liver GABA transaminase. Biomed Res Int 2014:590581 (2014). WB ; Rat . PubMed: 24809054
- Jo S et al. GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's disease. Nat Med N/A:N/A (2014). Mouse . PubMed: 24973918