Recombinant Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
![Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)](https://www.abcam.com/ps/products/197/ab197013/Images/ab197013-236607-anti-abcd1-ald-antibody-epr15929-western-blot.jpg "Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15929] to ABCD1/ALD
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ABCD1/ALD antibody [EPR15929]
See all ABCD1/ALD primary antibodies -
Description
Rabbit monoclonal [EPR15929] to ABCD1/ALD -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 cells, HeLa cells, human fetal liver lysate; JAR cells; C6 cells, RAW 264.7 cells, PC-12 cells, NIH/3t3 cells. ICC/IF: MCF7 cells. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15929 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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| ICC/IF |
1/100.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
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ICC/IF
1/100. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity. -
Involvement in disease
Defects in ABCD1 are the cause of adrenoleukodystrophy X-linked (X-ALD) [MIM:300100]. X-ALD is a peroxisomal metabolic disorder characterized by progressive multifocal demyelination of the central nervous system and by peripheral adrenal insufficiency (Addison disease). It results in mental deterioration, corticospinal tract dysfunction, and cortical blindness. Different clinical manifestations exist like: cerebral childhood ALD (CALD), adult cerebral ALD (ACALD), adrenomyeloneuropathy (AMN) and 'Addison disease only' (ADO) phenotype.
Note=The promoter region of ABCD1 is deleted in the chromosome Xq28 deletion syndrome which involves ABCD1 and the neighboring gene BCAP31. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain. -
Cellular localization
Peroxisome membrane. - Information by UniProt
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Database links
- Entrez Gene: 215 Human
- Entrez Gene: 11666 Mouse
- Entrez Gene: 363516 Rat
- Omim: 300371 Human
- SwissProt: P33897 Human
- SwissProt: P48410 Mouse
- Unigene: 159546 Human
- Unigene: 365 Mouse
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Alternative names
- ABC42 antibody
- Abcd1 antibody
- ABCD1_HUMAN antibody
see all
Images
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Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)All lanes : Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)](https://www.abcam.com/ps/products/197/ab197013/Images/ab197013-236605-anti-abcd1-ald-antibody-epr15929-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ABCD1 / ALD with ab197013 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab197013 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Flow Cytometry (Intracellular) - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)](https://www.abcam.com/ps/products/197/ab197013/Images/ab197013-263929-anti-abcd1-ald-antibody-epr15929-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)ab197013 staining ABCD1/ ALD in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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![Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)](https://www.abcam.com/ps/products/197/ab197013/Images/ab197013-236608-anti-abcd1-ald-antibody-epr15929-western-blot.jpg)
Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution + JAR (Human placenta choriocarcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)](https://www.abcam.com/ps/products/197/ab197013/Images/ab197013-236609-anti-abcd1-ald-antibody-epr15929-western-blot.jpg)
Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)All lanes : Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-ABCD1/ALD antibody [EPR15929] (ab197013)](https://www.abcam.com/ps/products/197/ab197013/Images/ab197013-6-benefits-of-recombinant-antibodies.png)
Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab197013 has been referenced in 4 publications.
- Gong Y et al. Peroxisome Metabolism Contributes to PIEZO2-Mediated Mechanical Allodynia. Cells 11:N/A (2022). PubMed: 35681537
- Kuramochi Y et al. Generation of two human induced pluripotent stem cell lines derived from two X-linked adrenoleukodystrophy patients with ABCD1 mutations. Stem Cell Res 53:102337 (2021). PubMed: 33901816
- Gong Y et al. Intrathecal Adeno-Associated Viral Vector-Mediated Gene Delivery for Adrenomyeloneuropathy. Hum Gene Ther 30:544-555 (2019). PubMed: 30358470
- Chang CL et al. Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. J Cell Biol 218:2583-2599 (2019). PubMed: 31227594