Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)
![Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)](https://www.abcam.com/ps/products/110/ab110289/Images/ab110289-123514-anti-acaa1-antibody-2h6be7-western-blot.jpg "Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)")
Key features and details
- Mouse monoclonal [2H6BE7] to ACAA1/Beta-ketothiolase
- Suitable for: ICC, Flow Cyt, IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7]
See all ACAA1/Beta-ketothiolase primary antibodies -
Description
Mouse monoclonal [2H6BE7] to ACAA1/Beta-ketothiolase -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cyt, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HepG2,HL-60 cells, human liver tissue, rat liver tissue and mouse liver tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
2H6BE7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 5 µg/ml.
Heat-induced antigen retrieval improves signal |
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IP |
Use a concentration of 5 µg/ml.
|
|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
|
| Notes |
|---|
|
ICC
Use a concentration of 5 µg/ml. Heat-induced antigen retrieval improves signal |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IP
Use a concentration of 5 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. |
Target
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Pathway
Lipid metabolism; fatty acid metabolism. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Entrez Gene: 24157 Rat
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
- SwissProt: P07871 Rat
- SwissProt: P21775 Rat
see all -
Alternative names
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
- ACAA antibody
see all
Images
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Western blot - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)All lanes : Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289) at 1 µg/ml
Lane 1 : Human liver
Lane 2 : HepG2
Lane 3 : Rat liver
Lane 4 : Mouse liver
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa -
![Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)](https://www.abcam.com/ps/products/110/ab110289/Images/ab110289-234731-anti-acaa1-antibody-2h6be7-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)ACAA1/Beta-ketothiolase was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Mouse monoclonal to ACAA1/Beta-ketothiolase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 44kDa; ACAA1/Beta-ketothiolase
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![Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)](https://www.abcam.com/ps/products/110/ab110289/Images/ab110289-123516-anti-acaa1-antibody-2h6be7-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)ab110289, at 5 µg/mL, staining ACAA1/Beta-ketothiolase in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).
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![Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)](https://www.abcam.com/ps/products/110/ab110289/Images/ab110289-123515-anti-acaa1-antibody-2h6be7-flow-cytometry.jpg)
Flow Cytometry - Anti-ACAA1/Beta-ketothiolase antibody [2H6BE7] (ab110289)ab110289, at 1 µg/mL, staining ACAA1/Beta-ketothiolase in HL-60 cells (blue) or using an isotype control (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab110289 has been referenced in 4 publications.
- Uzor NE et al. Aging lowers PEX5 levels in cortical neurons in male and female mouse brains. Mol Cell Neurosci 107:103536 (2020). PubMed: 32777345
- Xue Y et al. Maternal undernutrition induces fetal hepatic lipid metabolism disorder and affects the development of fetal liver in a sheep model. FASEB J 33:9990-10004 (2019). PubMed: 31167079
- Zhang S et al. hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC. Oncotarget 8:104508-104524 (2017). PubMed: 29262657
- Tomilov A et al. Shc depletion stimulates brown fat activity in vivo and in vitro. Aging Cell 13:1049-58 (2014). Mouse . PubMed: 25257068