Anti-ACADL/LCAD antibody [7F5DD6] (ab128566)
Key features and details
- Mouse monoclonal [7F5DD6] to ACADL/LCAD
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
-
Product name
Anti-ACADL/LCAD antibody [7F5DD6]
See all ACADL/LCAD primary antibodies -
Description
Mouse monoclonal [7F5DD6] to ACADL/LCAD -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
Positive control
- Rat Liver Homogenate, Rat Liver Mitochondria, Mouse Liver Homogenate and Mouse Liver Mitochondria
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
-
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
7F5DD6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
|
|
IP |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. |
IP
Use at an assay dependent concentration. |
Target
-
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
Involvement in disease
Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD) [MIM:201475]. An inborn error of mitochondrial fatty acid beta-oxidation which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form characterized by early onset, high mortality and high incidence of cardiomyopathy; a milder childhood form with later onset, characterized by hypoketotic hypoglycemia, low mortality and rare cardiomyopathy; an adult form, with isolated skeletal muscle involvement, rhabdomyolysis and myoglobinuria, usually triggered by exercise or fasting. -
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 11363 Mouse
- Entrez Gene: 25287 Rat
- SwissProt: P51174 Mouse
- SwissProt: P15650 Rat
- Unigene: 2445 Mouse
- Unigene: 174 Rat
-
Alternative names
- ACAD4 antibody
- ACADL antibody
- ACADL_HUMAN antibody
see all
Images
-
All lanes : Anti-ACADL/LCAD antibody [7F5DD6] (ab128566) at 1 µg/ml
Lane 1 : Ladder
Lane 2 : Rat Liver Homogenate at 15 µg
Lane 3 : Rat Liver Mitochondria at 15 µg
Lane 4 : Rat H4IIE cell lysate at 15 µg
Lane 5 : Mouse Liver Homogenate at 15 µg
Lane 6 : Mouse Liver Mitochondria at 15 µg
Lane 7 : Mouse MEF cell lysate at 15 µg
Predicted band size: 47 kDa -
Immunoprecipitation using ab128566
Lane 1: Ladder Lane 2: Rat Liver Homogenate.
Protocols
Datasheets and documents
-
Datasheet download
References (3)
ab128566 has been referenced in 3 publications.
- Laudette M et al. Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity. Cell Death Dis 12:824 (2021). PubMed: 34471096
- Maurya SK et al. Sarcolipin Signaling Promotes Mitochondrial Biogenesis and Oxidative Metabolism in Skeletal Muscle. Cell Rep 24:2919-2931 (2018). PubMed: 30208317
- Zhang R et al. Short-term administration of Nicotinamide Mononucleotide preserves cardiac mitochondrial homeostasis and prevents heart failure. J Mol Cell Cardiol 112:64-73 (2017). PubMed: 28882480