Anti-ACADM/MCAD antibody [3B7BH7] (ab110296)
Key features and details
- Mouse monoclonal [3B7BH7] to ACADM/MCAD
- Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-ACADM/MCAD antibody [3B7BH7]
See all ACADM/MCAD primary antibodies -
Description
Mouse monoclonal [3B7BH7] to ACADM/MCAD -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells, HL-60 cells, Human cerebellum, HepG2 cells, HeLa cells, H9C2 (rat cells), and MEF (mouse cells) lysates.
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General notes
MCAD (medium-chain acyl-CoA dehydrogenase) is an oxidoreductase enzyme of the mitochondrial fatty acid beta-oxidation pathway that is specific for acyl chain lengths of 4 to 16. It also utilizes the electron transfer flavoprotein (ETF) as electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Primary antibody notes
MCAD (medium-chain acyl-CoA dehydrogenase) is an oxidoreductase enzyme of the mitochondrial fatty acid beta-oxidation pathway that is specific for acyl chain lengths of 4 to 16. It also utilizes the electron transfer flavoprotein (ETF) as electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). -
Clonality
Monoclonal -
Clone number
3B7BH7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.125 µg/ml. Predicted molecular weight: 47 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 0.125 µg/ml. Predicted molecular weight: 47 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
This enzyme is specific for acyl chain lengths of 4 to 16. -
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
Involvement in disease
Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. -
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 34 Human
- Entrez Gene: 11364 Mouse
- Entrez Gene: 24158 Rat
- Omim: 607008 Human
- SwissProt: P11310 Human
- SwissProt: P45952 Mouse
- SwissProt: P08503 Rat
- Unigene: 445040 Human
see all -
Alternative names
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
see all
Images
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All lanes : Anti-ACADM/MCAD antibody [3B7BH7] (ab110296) at 0.125 µg/ml
Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : H9C2 (rat cells) lysates
Lane 4 : MEF (mouse cells) lysates
Lysates/proteins at 15 µg per lane.
Predicted band size: 47 kDa -
Immunocytochemistry image of ab110296 stained Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110296 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM/MCAD antibody [3B7BH7] (ab110296)
ACADM/MCAD immunohistochemistry in Human cerebellum visualized with ab110296 at 1/1000. MCAD immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of MCAD immunoreactivity in the Purkinje cell bodies.
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HL-60 cells were stained with 1 µg/mL ab110296 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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HL-60 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of fenofibrate (ab120832). Increased expression of ACADM/MCAD in HL-60 cells correlates with an increase in fenofibrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab110296 at 1 µg/ml and ab9484 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab110296 has been referenced in 12 publications.
- Zhao J et al. Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIV. Front Immunol 12:658420 (2021). PubMed: 34017335
- Hidalgo-Gutiérrez A et al. β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight. Biomedicines 9:N/A (2021). PubMed: 34680574
- Zhu L et al. Expressing the Human Cholesteryl Ester Transfer Protein Minigene Improves Diet-Induced Fatty Liver and Insulin Resistance in Female Mice. Front Physiol 12:799096 (2021). PubMed: 35082691
- Gao K et al. Qishen granules exerts cardioprotective effects on rats with heart failure via regulating fatty acid and glucose metabolism. Chin Med 15:21 (2020). PubMed: 32158496
- Zhou C et al. Gadofullerene inhibits the degradation of apolipoprotein B100 and boosts triglyceride transport for reversing hepatic steatosis. Sci Adv 6:N/A (2020). PubMed: 32917715