Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)
![Western blot - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)](https://www.abcam.com/ps/products/110/ab110318/Images/ACADS-Primary-antibodies-ab110318-1.gif "Western blot - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)")
Key features and details
- Mouse monoclonal [7E1AB5] to ACADS/SCAD
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-ACADS/SCAD antibody [7E1AB5]
See all ACADS/SCAD primary antibodies -
Description
Mouse monoclonal [7E1AB5] to ACADS/SCAD -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Fibroblast, HeLa, HepG2. Human Heart FFPE tissue.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
7E1AB5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. |
Target
-
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
Involvement in disease
Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults. -
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 35 Human
- Omim: 606885 Human
- SwissProt: P16219 Human
- Unigene: 507076 Human
-
Alternative names
- ACAD3 antibody
- ACADS antibody
- ACADS_HUMAN antibody
see all
Images
-
Western blot - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)All lanes : Anti-ACADS/SCAD antibody [7E1AB5] (ab110318) at 1 µg/ml
Lane 1 : HepG2 whole cell cells at 10 µg
Lane 2 : ACADS at 0.01 µg
Predicted band size: 44 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)](https://www.abcam.com/ps/products/110/ab110318/Images/ACADS-Primary-antibodies-ab110318-3.gif)
Immunocytochemistry/ Immunofluorescence - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)Immunocytochemistry image of stained human fibroblasts. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110318 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)](https://www.abcam.com/ps/products/110/ab110318/Images/ACADS-Primary-antibodies-ab110318-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [7E1AB5] (ab110318)IHC image of ACADS/SCAD staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110318, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab110318 has not yet been referenced specifically in any publications.
