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Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)

Submit a review Q&A (2)References (1)
Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
  • Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)

Key features and details

  • Mouse monoclonal [6A9AF2] to ACADVL/VLCAD
  • Suitable for: ICC/IF, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ACADVL/VLCAD antibody [6A9AF2]
    See all ACADVL/VLCAD primary antibodies

  • Description

    Mouse monoclonal [6A9AF2] to ACADVL/VLCAD

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Human HDFn cells; Human liver mitochondria; Human cerebellum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
IP
Use at an assay dependent concentration.
IHC-P
1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
IP
Use at an assay dependent concentration.
IHC-P
1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.

Target

  • Function

    Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.

  • Pathway

    Lipid metabolism; mitochondrial fatty acid beta-oxidation.

  • Involvement in disease

    Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD) [MIM:201475]. ACADVLD is an autosomal recessive disease which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form, with early onset, high mortality, and high incidence of cardiomyopathy; a milder childhood form, with later onset, usually with hypoketotic hypoglycemia as the main presenting feature, low mortality, and rare cardiomyopathy; and an adult form, with isolated skeletal muscle involvement, rhabdomyolysis, and myoglobinuria, usually triggered by exercise or fasting.

  • Sequence similarities

    Belongs to the acyl-CoA dehydrogenase family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ACAD 6 antibody
      • ACAD6 antibody
      • ACADV_HUMAN antibody
      • Acadvl antibody
      • Acyl CoA dehydrogenase very long chain antibody
      • Acyl Coenzyme A dehydrogenase very long chain antibody
      • LCACD antibody
      • mitochondrial antibody
      • Very long chain specific acyl CoA dehydrogenase antibody
      • Very long chain specific acyl CoA dehydrogenase mitochondrial antibody
      • Very long-chain specific acyl-CoA dehydrogenase antibody
      • VLCAD antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
      Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)

      Immunocytochemistry image of ACADVL/VLCAD antibody [6A9AF2] (ab110285) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110285 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)

      ab110285,at 1/250 dilution, staining ACADVL/VLCAD in formalin-fixed, paraffin-embedded human cerebellum tissue. Immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

    • Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)
      Immunoprecipitation - Anti-ACADVL/VLCAD antibody [6A9AF2] (ab110285)

      ACADVL/VLCAD immunocaptured from 0.75mg of human liver mitochondria lysate /10ul antibody conjugated beads using ab110285.

    References (1)

    Publishing research using ab110285? Please let us know so that we can cite the reference in this datasheet.

    ab110285 has been referenced in 1 publication.

    • Sweeney TE  et al. A toll-like receptor 2 pathway regulates the Ppargc1a/b metabolic co-activators in mice with Staphylococcal aureus sepsis. PLoS One 6:e25249 (2011). WB ; Mouse . PubMed: 21966468

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    How does the abcam trial program work?

    Read More
    Answer

    Thank you for your reply.

    For UNTESTED applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested.

    To take part in our AbTrial Program:

    1. Contact our Scientific Support group to determine whether this product is eligible for enrollment in the AbTrial program at us.scientificsupport@abcam.com

    2. If the product is eligible, we will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at regular price.

    4. https://www.abcam.com/abreviews with your results. Note your AbTrial discount code in the additional notes section.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

    I hope this information is helpful. Please do not hesitate to contact us if you have any questions.

    Read More

    Question

    Thanks for your help on the phone tonight, with my questions about the Anti-VLCAD antibody from Abcam (Mito Sciences), AB110285. We would like to find out where we expect to find distribution within mitochondria by thin-section TEM (LR-White Resin) in mitochondria that are apposed to other organelles in cells. Does one expect to see linear arrays of immunogold or Fluoronanogold (secondary antibody) probes along the inner membrane, or close to the membranes of the other organelles in contact with the mitochondria?   Our experiment did not work with the VLCAD for either Santa Cruz' sample or your sample in heart. We use Fluoronanogold, so it did not show up by confocal or TEM. I appreciate your help.

    Read More
    Answer

    Thank you for contacting us. I wish to apologize for the wait.

    We really do not know what you would expect to see with this product in EM, e.g. linear arrays probes along the inner membrane. Regarding whether this product should work with gold conjugation, we have not yet tried this but in our experience some Abs work really well, while others do not regardless of conjugation conditions.

    I am very sorry that I could not be more helpful in this case. If you are interested in the AbTrial program please let me know. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

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