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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

Reviews (1) Submit a question
Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
  • Flow Cytometry (Intracellular) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
  • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
  • Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
  • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
  • Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15107(B)] to ACADVL/VLCAD
  • Suitable for: Flow Cyt (Intra), IP, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ACADVL/VLCAD antibody [EPR15107(B)]
    See all ACADVL/VLCAD primary antibodies

  • Description

    Rabbit monoclonal [EPR15107(B)] to ACADVL/VLCAD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, A431 and 293 cell lysates. IHC-P: Human kidney tissue and colon adenocarcinoma tissue. WB: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/40.
WB
1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 70 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/40.
WB
1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 70 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.

  • Pathway

    Lipid metabolism; mitochondrial fatty acid beta-oxidation.

  • Involvement in disease

    Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD) [MIM:201475]. ACADVLD is an autosomal recessive disease which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form, with early onset, high mortality, and high incidence of cardiomyopathy; a milder childhood form, with later onset, usually with hypoketotic hypoglycemia as the main presenting feature, low mortality, and rare cardiomyopathy; and an adult form, with isolated skeletal muscle involvement, rhabdomyolysis, and myoglobinuria, usually triggered by exercise or fasting.

  • Sequence similarities

    Belongs to the acyl-CoA dehydrogenase family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ACAD 6 antibody
      • ACAD6 antibody
      • ACADV_HUMAN antibody
      • Acadvl antibody
      • Acyl CoA dehydrogenase very long chain antibody
      • Acyl Coenzyme A dehydrogenase very long chain antibody
      • LCACD antibody
      • mitochondrial antibody
      • Very long chain specific acyl CoA dehydrogenase antibody
      • Very long chain specific acyl CoA dehydrogenase mitochondrial antibody
      • Very long-chain specific acyl-CoA dehydrogenase antibody
      • VLCAD antibody
      see all

    Images

    • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      All lanes : Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : ACADVL knockout HEK293T cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 70 kDa
      Observed band size: 70 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab188872 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab188872 Anti-ACADVL/VLCAD antibody [EPR15107(B)] was shown to specifically react with ACADVL/VLCAD in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266484 (knockout cell lysate ab257332) was used. Wild-type and ACADVL/VLCAD knockout samples were subjected to SDS-PAGE. ab188872 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Flow Cytometry (Intracellular) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

      Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ACADVL/VLCAD with ab188872 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

      Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling ACADVL/VLCAD with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACADVL/VLCAD with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/1000 dilution + 293 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 70 kDa

    • Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

      Western blot analysis of immunoprecipitation pellet from A431 lysate immunoprecipitated using ab188872 at 1/50 dilution.

      Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      All lanes : Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 70 kDa

    • Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
      Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    References (0)

    Publishing research using ab188872? Please let us know so that we can cite the reference in this datasheet.

    ab188872 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-ACADVL/VLCAD antibody [EPR15107(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Cardiomyocytes)
    Permeabilization
    Yes - Triton-100
    Specification
    Cardiomyocytes
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 28 2016

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