Anti-ACAT1 antibody [9H10AB4] (ab110290)
![Immunocytochemistry - Anti-ACAT1 antibody [9H10AB4] (ab110290)](https://www.abcam.com/ps/products/110/ab110290/Images/ab110290-123520-anti-acat1-antibody-9h10ab4-immunocytochemistry.gif "Immunocytochemistry - Anti-ACAT1 antibody [9H10AB4] (ab110290)")
Key features and details
- Mouse monoclonal [9H10AB4] to ACAT1
- Suitable for: ICC, Flow Cyt, IP, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-ACAT1 antibody [9H10AB4]
See all ACAT1 primary antibodies -
Description
Mouse monoclonal [9H10AB4] to ACAT1 -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cyt, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- ICC: Human HDFn cells IHC-P: Human cerebellumIP: HepG2 cells and Human liver mitochondria Flow Cyt: HeLa cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
9H10AB4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 5 µg/ml.
|
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval via the pressure cooker method (1 minute, with 1 mmol EDTA at pH8) before commencing with IHC staining protocol.
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| Notes |
|---|
|
ICC
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. |
|
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method (1 minute, with 1 mmol EDTA at pH8) before commencing with IHC staining protocol. |
Target
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Function
Plays a major role in ketone body metabolism. -
Involvement in disease
Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 38 Human
- Omim: 607809 Human
- SwissProt: P24752 Human
- Unigene: 232375 Human
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Alternative names
- ACAT 1 antibody
- ACAT antibody
- acat1 antibody
see all
Images
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Immunocytochemistry - Anti-ACAT1 antibody [9H10AB4] (ab110290)Immunocytochemistry image of ab110290 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody (9H10AB4, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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![Immunoprecipitation - Anti-ACAT1 antibody [9H10AB4] (ab110290)](https://www.abcam.com/ps/products/110/ab110290/Images/ab110290-123519-anti-acat1-antibody-9h10ab4-immunoprecipitation.gif)
Immunoprecipitation - Anti-ACAT1 antibody [9H10AB4] (ab110290)ab110290 immunocaptured from HepG2 cells (lane 1) and Human liver mitochondria (lane 2)
Predicted molecular weight is 44 kDa. -
![Flow Cytometry - Anti-ACAT1 antibody [9H10AB4] (ab110290)](https://www.abcam.com/ps/products/110/ab110290/Images/ab110290-123518-anti-acat1-antibody-9h10ab4-flow-cytometry.gif)
Flow Cytometry - Anti-ACAT1 antibody [9H10AB4] (ab110290)ab110290, at 1 µg/mL, staining ACAT1 in HeLa (blue) or in an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [9H10AB4] (ab110290)](https://www.abcam.com/ps/products/110/ab110290/Images/ab110290-123517-anti-acat1-antibody-9h10ab4-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [9H10AB4] (ab110290)ACAT1 immunohistochemistry in human cerebellum visualized with ab110290. ACAT1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of ACAT1 immunoreactivity in the Purkinje cell bodies. The functional significance of this pattern is unknown at present but this antibody offers the opportunity to investigate it in more detail.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab110290 has been referenced in 1 publication.
- Guan J et al. Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1. J Cell Mol Med 24:14039-14049 (2020). PubMed: 33103371