Recombinant Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)
![Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)](https://www.abcam.com/ps/products/131/ab131215/Images/ab131215-145517-anti-acetyl-coa-acetyltransferase-antibody-epr8417-western-blot.jpg "Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417]
See all ACAT2/Acetyl-CoA acetyltransferase primary antibodies -
Description
Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human ACAT2/Acetyl-CoA acetyltransferase aa 200-300. The exact sequence is proprietary.
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Positive control
- Human liver tissue; HepG2, HeLa, Jurkat and K562 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.91 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8417 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 mins. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 mins. |
Application notes
Is unsuitable for ICC/IF.
Target
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Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 39 Human
- Entrez Gene: 110460 Mouse
- Entrez Gene: 308100 Rat
- Omim: 100678 Human
- SwissProt: Q9BWD1 Human
- SwissProt: Q8CAY6 Mouse
- SwissProt: Q5XI22 Rat
- Unigene: 571037 Human
see all -
Alternative names
- Acat2 antibody
- acetoacetyl CoA thiolase antibody
- acetoacetyl Coenzyme A thiolase antibody
see all
Images
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Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)](https://www.abcam.com/ps/products/131/ab131215/Images/ab131215-145516-anti-acetyl-coa-acetyltransferase-antibody-epr8417-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)Immunohistochemical analysis of paraffin embedded Human liver tissue labelling ACAT2/Acetyl-CoA acetyltransferase with ab131215 antibody at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)](https://www.abcam.com/ps/products/131/ab131215/Images/ab131215-145515-anti-acetyl-coa-acetyltransferase-antibody-epr8417.jpg)
OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) -
![Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)](https://www.abcam.com/ps/products/131/ab131215/Images/ab131215-4-benefits-of-recombinant-antibodies.png)
Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab131215 has been referenced in 7 publications.
- Vergani E et al. Targeting of the Lipid Metabolism Impairs Resistance to BRAF Kinase Inhibitor in Melanoma. Front Cell Dev Biol 10:927118 (2022). PubMed: 35912092
- Wang S et al. Role of sortilin 1 (SORT1) on fatty acid-mediated cholesterol metabolism in primary calf hepatocytes. J Dairy Sci 105:7773-7786 (2022). PubMed: 35940922
- Buettner R et al. Targeting the metabolic vulnerability of acute myeloid leukemia blasts with a combination of venetoclax and 8-chloro-adenosine. J Hematol Oncol 14:70 (2021). PubMed: 33902674
- Zhang Z et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
- Cheng L et al. Circ_RPL23A acts as a miR-1233 sponge to suppress the progression of clear cell renal cell carcinoma by promoting ACAT2. J Bioenerg Biomembr 53:415-428 (2021). PubMed: 34036483
- Tian F et al. MiR-542-5p Inhibits Hyperglycemia and Hyperlipoidemia by Targeting FOXO1 in the Liver. Yonsei Med J 61:780-788 (2020). PubMed: 32882762
- Weng M et al. ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer. Onco Targets Ther 13:3477-3488 (2020). PubMed: 32425549