Recombinant Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free
See all ACAT2/Acetyl-CoA acetyltransferase primary antibodies -
Description
Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248375 is the carrier-free version of ab131215.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.91 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8417 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
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Target
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Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 39 Human
- Entrez Gene: 110460 Mouse
- Entrez Gene: 308100 Rat
- Omim: 100678 Human
- SwissProt: Q9BWD1 Human
- SwissProt: Q8CAY6 Mouse
- SwissProt: Q5XI22 Rat
- Unigene: 571037 Human
see all -
Alternative names
- Acat2 antibody
- acetoacetyl CoA thiolase antibody
- acetoacetyl Coenzyme A thiolase antibody
see all
Images
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Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDaThis data was developed using ab131215, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)This data was developed using ab131215, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human liver tissue labelling ACAT2/Acetyl-CoA acetyltransferase with ab131215 antibody at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)This data was developed using ab131215, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab248375 has been referenced in 1 publication.
- Qiu H et al. Regulation and Mechanism of miR-518d through the PPARa-Mediated NF-?B Pathway in the Development of Gestational Diabetes Mellitus. J Diabetes Res 2020:7019597 (2020). PubMed: 33123597