Recombinant Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
![Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)](https://www.abcam.com/ps/products/248/ab248750/Images/ab248750-407996-anti-acbd3-antibody-epr8623-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8623] to ACBD3 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ACBD3 antibody [EPR8623] - BSA and Azide free
See all ACBD3 primary antibodies -
Description
Rabbit monoclonal [EPR8623] to ACBD3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248750 is the carrier-free version of ab134952.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.28 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8623 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa. |
Target
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Function
Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells. -
Tissue specificity
Ubiquitous, with highest expression in testis and ovary. -
Sequence similarities
Contains 1 ACB (acyl-CoA-binding) domain.
Contains 1 GOLD domain. -
Domain
The GOLD domain is essential for giantin binding. -
Cellular localization
Golgi apparatus membrane. Mitochondrion. Also mitochondrial (via its interaction with PBR). - Information by UniProt
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Database links
- Entrez Gene: 64746 Human
- Omim: 606809 Human
- SwissProt: Q9H3P7 Human
- Unigene: 520207 Human
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Alternative names
- ACBD 3 antibody
- ACBD3 antibody
- Acyl CoA binding domain containing protein 3 antibody
see all
Images
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Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
This data was developed using ab134952, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ACBD3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab134952 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab134952 was shown to specifically react with ACBD3 when ACBD3 knockout samples were used. Wild-type and ACBD3 knockout samples were subjected to SDS-PAGE. ab134952 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)](https://www.abcam.com/ps/products/248/ab248750/Images/ab248750-406207-anti-acbd3-antibody-epr8623-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)All lanes : Anti-ACBD3 antibody [EPR8623] (ab134952) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : LNCaP cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 61 kDaThis data was developed using ab134952, the same antibody clone in a different buffer formulation.
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![Flow Cytometry (Intracellular) - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)](https://www.abcam.com/ps/products/248/ab248750/Images/ab248750-2-anti-acbd3-antibody-epr8623-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750) -
![OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)](https://www.abcam.com/ps/products/248/ab248750/Images/ab248750-1-anti-acbd3-antibody-epr8623-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)This data was developed using ab134952, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)](https://www.abcam.com/ps/products/248/ab248750/Images/ab248750-5-benefits-of-recombinant-antibodies.png)
Anti-ACBD3 antibody [EPR8623] - BSA and Azide free (ab248750)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248750 has not yet been referenced specifically in any publications.
