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    products/primary-antibodies/ace2-antibody-mm0073-11a31-ab89111.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
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Anti-ACE2 antibody [MM0073-11A31] (ab89111)

  • Datasheet
Reviews (4) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [MM0073-11A31] (ab89111)

    Key features and details

    • Mouse monoclonal [MM0073-11A31] to ACE2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2

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    Overview

    • Product name

      Anti-ACE2 antibody [MM0073-11A31]
      See all ACE2 primary antibodies
    • Description

      Mouse monoclonal [MM0073-11A31] to ACE2
    • Host species

      Mouse
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment corresponding to Human ACE2 (extracellular).
      Database link: Q9BYF1

    • Positive control

      • WB: Human kidney tissue lysate. IHC-P: Human kidney tissue.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      MM0073-11A31
    • Isotype

      IgG2
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Metalloprotease
      • ACEs
      • Cardiovascular
      • Blood
      • Blood Pressure regulation
      • Cardiovascular
      • Heart
      • Contractility
      • Inotropics
      • Cardiovascular
      • Vasculature
      • Vasoconstriction
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Recombinant Protein

      • Recombinant Human ACE2 protein (ab151852)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab89111 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/50 - 1/200.
    Notes
    IHC-P
    1/50 - 1/200.

    Target

    • Function

      Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
    • Tissue specificity

      Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
    • Sequence similarities

      Belongs to the peptidase M2 family.
    • Post-translational
      modifications

      N-glycosylation on Asn-90 may limit SARS infectivity.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession Q9BYF1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 59272 Human
      • Omim: 300335 Human
      • SwissProt: Q9BYF1 Human
      • Unigene: 178098 Human
      • Alternative names

        • ACE 2 antibody
        • ACE related carboxypeptidase antibody
        • ACE-related carboxypeptidase antibody
        • ACE2 antibody
        • ACE2_HUMAN antibody
        • ACEH antibody
        • Angiotensin converting enzyme 2 antibody
        • Angiotensin converting enzyme homolog antibody
        • Angiotensin converting enzyme like protein antibody
        • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
        • Angiotensin I converting enzyme 2 antibody
        • Angiotensin-converting enzyme homolog antibody
        • DKFZP434A014 antibody
        • EC 3.4.17 antibody
        • metalloprotease MPROT 15 antibody
        • Metalloprotease MPROT15 antibody
        • OTTHUMP00000022963 antibody
        • Processed angiotensin-converting enzyme 2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [MM0073-11A31] (ab89111)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [MM0073-11A31] (ab89111)

        IHC image of ACE2 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab89111, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (3)

      Publishing research using ab89111? Please let us know so that we can cite the reference in this datasheet.

      ab89111 has been referenced in 3 publications.

      • McAlinden KD  et al. Electronic Cigarette Aerosol Is Cytotoxic and Increases ACE2 Expression on Human Airway Epithelial Cells: Implications for SARS-CoV-2 (COVID-19). J Clin Med 10:N/A (2021). PubMed: 33802256
      • Mencucci R  et al. Co-expression of the SARS-CoV-2 entry receptors ACE2 and TMPRSS2 in healthy human conjunctiva. Exp Eye Res 205:108527 (2021). PubMed: 33667466
      • Liang Y  et al. Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model. Zool Res 41:621-631 (2020). PubMed: 33045777

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [MM0073-11A31]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (Rat hepatocytes)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Rat hepatocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Armen Petrosyan

      Verified customer

      Submitted Sep 08 2020

      Western blot abreview for Anti-ACE2 antibody [MM0073-11A31]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (Rat hepatocytes)
      Gel Running Conditions
      Reduced Denaturing (8% SDS-PAGE)
      Loading amount
      30 µg
      Specification
      Rat hepatocytes
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Jul 27 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [MM0073-11A31]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Human Alveolar Epithelial Cell)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Human Alveolar Epithelial Cell
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Armen Petrosyan

      Verified customer

      Submitted Jul 24 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [MM0073-11A31]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Mice hepatocytes)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Mice hepatocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Jul 23 2020

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