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    products/primary-antibodies/acetyl-coenzyme-a-carboxylase-phospho-s79-antibody-ep1885y-ab68191.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (24)

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Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
  • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
  • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
  • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
  • Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
  • Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79)
  • Suitable for: Dot blot, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Assay
Product image
D-Lactate Assay Kit (Fluorometric) (ab174096)
Protein
Product image
Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description

    Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 cell lysate, PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (treated and untreated), RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate (treated and untreated).
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1885Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Synthesis
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
WB (1)
1/5000 - 1/10000. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
Notes
Dot blot
Use at an assay dependent concentration.
WB
1/5000 - 1/10000. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
  • Tissue specificity

    Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
  • Pathway

    Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
  • Involvement in disease

    Acetyl-CoA carboxylase 1 deficiency
  • Sequence similarities

    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • Post-translational
    modifications

    Phosphorylation on Ser-1263 is required for interaction with BRCA1.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q13085 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 31 Human
    • Entrez Gene: 32 Human
    • Entrez Gene: 100705 Mouse
    • Entrez Gene: 107476 Mouse
    • Entrez Gene: 116719 Rat
    • Entrez Gene: 60581 Rat
    • Omim: 200350 Human
    • Omim: 601557 Human
    • SwissProt: O00763 Human
    • SwissProt: Q13085 Human
    • SwissProt: O00763 Mouse
    • SwissProt: Q5SWU9 Mouse
    • SwissProt: O70151 Rat
    • SwissProt: P11497 Rat
    • Unigene: 160556 Human
    • Unigene: 234898 Human
    • Unigene: 31374 Mouse
    • Unigene: 163753 Rat
    • Unigene: 217177 Rat
    • Unigene: 44372 Rat
    see all
  • Alternative names

    • ACAC antibody
    • ACACA antibody
    • ACACA_HUMAN antibody
    • ACACB antibody
    • ACC alpha antibody
    • ACC antibody
    • ACC beta antibody
    • ACC-alpha antibody
    • ACC1 antibody
    • ACC2 antibody
    • ACCA antibody
    • ACCB antibody
    • Acetyl CoA carboxylase 1 antibody
    • Acetyl CoA carboxylase 2 antibody
    • Acetyl CoA carboxylase alpha antibody
    • Acetyl CoA carboxylase beta antibody
    • Acetyl Coenzyme A carboxylase alpha antibody
    • Acetyl Coenzyme A carboxylase beta antibody
    • Biotin carboxylase antibody
    • COA1 antibody
    • COA2 antibody
    • HACC275 antibody
    • OTTHUMP00000164069 antibody
    • OTTHUMP00000164070 antibody
    • OTTHUMP00000164076 antibody
    • OTTHUMP00000240532 antibody
    see all

Images

  • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ACACA knockout HAP1 whole cell lysate
    Lane 3 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 277 kDa
    Observed band size: 266 kDa why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab68191 observed at 266 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab68191 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ACACA knockout cells. Wild-type and ACACA knockout samples were subjected to SDS-PAGE. Ab68191 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate. Then the membrane was incubated with phosphatase.
    Lane 2 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 277 kDa
    Observed band size: 265 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution

    Lane 1 : RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate. Then the membrane was incubated with phosphatase.
    Lane 2 : Untreated RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 277 kDa
    Observed band size: 265 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/10000 dilution

    Lane 1 : Untreated A431 whole cell lysate
    Lane 2 : A431 whole cell lysate - treated with lambda phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution

    Predicted band size: 277 kDa
    Observed band size: 265 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

    Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
    Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (24)

Publishing research using ab68191? Please let us know so that we can cite the reference in this datasheet.

ab68191 has been referenced in 24 publications.

  • Wang D  et al. FGF1?HBS prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppression. Signal Transduct Target Ther 6:133 (2021). PubMed: 33762571
  • Wang L  et al. Adropin inhibits the phenotypic modulation and proliferation of vascular smooth muscle cells during neointimal hyperplasia by activating the AMPK/ACC signaling pathway. Exp Ther Med 21:560 (2021). PubMed: 33850532
  • Yuan S  et al. Amelioration of the Lipogenesis, Oxidative Stress and Apoptosis of Hepatocytes by a Novel Proteoglycan from Ganoderma lucidum. Biol Pharm Bull 43:1542-1550 (2020). PubMed: 32759548
  • Da Eira D  et al. Effects of the adiponectin mimetic compound ALY688 on glucose and fat metabolism in visceral and subcutaneous rat adipocytes. Adipocyte 9:550-562 (2020). PubMed: 32897149
  • Li M  et al. Sirt3 modulates fatty acid oxidation and attenuates cisplatin-induced AKI in mice. J Cell Mol Med 24:5109-5121 (2020). PubMed: 32281286
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3-L1 mature adipocytes)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
15 µg
Treatment
0.1% DMSO, AICAR 2mM
Specification
3T3-L1 mature adipocytes
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Janaiya Samuels

Verified customer

Submitted Apr 27 2018

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