Recombinant Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
![Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)](https://www.abcam.com/ps/products/68/ab68191/Images/ab68191-366535-anti-acetyl-coenzyme-a-carboxylase-phospho-s79-antibody-ep1885y-western-blot-knockout-hap1-empty-acaca-ko-human-lysates.jpg "Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79)
- Suitable for: Dot blot, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]
See all Acetyl Coenzyme A Carboxylase primary antibodies -
Description
Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 cell lysate, PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (treated and untreated), RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate (treated and untreated).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1885Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
Use at an assay dependent concentration.
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| WB | (1) |
1/5000 - 1/10000. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
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| Notes |
|---|
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Dot blot
Use at an assay dependent concentration. |
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WB
1/5000 - 1/10000. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa). |
Target
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Function
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. -
Tissue specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. -
Pathway
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
Involvement in disease
Acetyl-CoA carboxylase 1 deficiency -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
Post-translational
modificationsPhosphorylation on Ser-1263 is required for interaction with BRCA1. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Entrez Gene: 100705 Mouse
- Entrez Gene: 107476 Mouse
- Entrez Gene: 116719 Rat
- Entrez Gene: 60581 Rat
- Omim: 200350 Human
- Omim: 601557 Human
see all -
Alternative names
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
see all
Images
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Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ACACA knockout HAP1 whole cell lysate
Lane 3 : HEK293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 277 kDa
Observed band size: 266 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab68191 observed at 266 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab68191 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ACACA knockout cells. Wild-type and ACACA knockout samples were subjected to SDS-PAGE. Ab68191 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)](https://www.abcam.com/ps/products/68/ab68191/Images/ab68191-265990-Untitled5.jpg)
Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate. Then the membrane was incubated with phosphatase.
Lane 2 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 277 kDa
Observed band size: 265 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)](https://www.abcam.com/ps/products/68/ab68191/Images/ab68191-265989-Untitled4.jpg)
Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate. Then the membrane was incubated with phosphatase.
Lane 2 : Untreated RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 277 kDa
Observed band size: 265 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)](https://www.abcam.com/ps/products/68/ab68191/Images/ab68191-253316-ab68191WB.jpg)
Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/10000 dilution
Lane 1 : Untreated A431 whole cell lysate
Lane 2 : A431 whole cell lysate - treated with lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution
Predicted band size: 277 kDa
Observed band size: 265 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)](https://www.abcam.com/ps/products/68/ab68191/Images/ab68191-253315-ab68191dot.jpg)
Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)](https://www.abcam.com/ps/products/68/ab68191/Images/ab68191-7-benefits-of-recombinant-antibodies.png)
Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab68191 has been referenced in 30 publications.
- Li J et al. Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagy. Nat Commun 13:1172 (2022). PubMed: 35246531
- Yu Y et al. Exercise-Generated β-Aminoisobutyric Acid (BAIBA) Reduces Cardiomyocyte Metabolic Stress and Apoptosis Caused by Mitochondrial Dysfunction Through the miR-208b/AMPK Pathway. Front Cardiovasc Med 9:803510 (2022). PubMed: 35282369
- Zheng Y et al. Nuclear receptor 4A1 (NR4A1) silencing protects hepatocyte against hypoxia-reperfusion injury in vitro by activating liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) signaling. Bioengineered 13:8349-8359 (2022). PubMed: 35311465
- Cheng L et al. Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels. Phytother Res 36:2495-2510 (2022). PubMed: 35445769
- Chen S et al. The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice. Front Physiol 12:709135 (2021). PubMed: 34588991