Anti-Acetylcholinesterase antibody [HR2] (ab2803)
Key features and details
- Mouse monoclonal [HR2] to Acetylcholinesterase
- Suitable for: ELISA, Flow Cyt, IHC-Fr, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rabbit, Guinea pig, Cow, Cat, Human, Macaque monkey
- Isotype: IgG2b
Overview
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Product name
Anti-Acetylcholinesterase antibody [HR2]
See all Acetylcholinesterase primary antibodies -
Description
Mouse monoclonal [HR2] to Acetylcholinesterase -
Host species
Mouse -
Specificity
This antibody does not detect butyrylcholinesterase (BChE). -
Tested applications
Suitable for: ELISA, Flow Cyt, IHC-Fr, IHC-P, ICC/IF, IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rabbit, Guinea pig, Cow, Cat, Human, Macaque monkey
Predicted to work with: Non human primatesDoes not react with: Rat, Amphibian
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Immunogen
Full length native protein (purified) corresponding to Human Acetylcholinesterase. Purified Human cerebellar acetylcholinesterase.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HR2 -
Isotype
IgG2b -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-Fr |
Use at an assay dependent concentration.
Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals. |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/100 - 1/1000.
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IP |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/1000. |
IP
Use at an assay dependent concentration. |
Target
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Function
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
Tissue specificity
Isoform H is highly expressed in erythrocytes. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Cellular localization
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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Database links
- Entrez Gene: 540446 Cow
- Entrez Gene: 43 Human
- Entrez Gene: 11423 Mouse
- Omim: 100740 Human
- SwissProt: P23795 Cow
- SwissProt: P22303 Human
- SwissProt: P21836 Mouse
- SwissProt: Q29499 Rabbit
see all -
Alternative names
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in HeLa cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in Neuro-2a cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in U251 cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Rectum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Overlay histogram showing HeLa cells stained with ab2803 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2803, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab2803 has been referenced in 12 publications.
- Singh M et al. 22(R)-hydroxycholesterol for dopaminergic neuronal specification of MSCs and amelioration of Parkinsonian symptoms in rats. Cell Death Discov 7:13 (2021). PubMed: 33454721
- Keck S et al. Lack of Mucosal Cholinergic Innervation Is Associated With Increased Risk of Enterocolitis in Hirschsprung's Disease. Cell Mol Gastroenterol Hepatol 12:507-545 (2021). PubMed: 33741501
- Horio T et al. Immunohistochemical analysis for acetylcholinesterase and choline acetyltransferase in mouse cerebral cortex after traumatic brain injury. J Vet Med Sci 82:827-835 (2020). PubMed: 32321871
- Sun Q et al. LncRNA-MALAT1 regulates proliferation and apoptosis of ovarian cancer cells by targeting miR-503-5p. Onco Targets Ther 12:6297-6307 (2019). PubMed: 31496733
- Dafferner AJ et al. Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure. Chem Res Toxicol 30:1897-1910 (2017). PubMed: 28892361