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Anti-Aconitase 2 antibody [6D1BE4] (ab110320)

Submit a review Q&A (1)
Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
  • Immunoprecipitation - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
  • Flow Cytometry - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)

Key features and details

  • Mouse monoclonal [6D1BE4] to Aconitase 2
  • Suitable for: IP, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Aconitase 2 antibody [6D1BE4]
    See all Aconitase 2 primary antibodies

  • Description

    Mouse monoclonal [6D1BE4] to Aconitase 2

  • Host species

    Mouse

  • Specificity

    This antibody is not suitable for Western blot. We recommend ab110321 for Western blotting.

  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cytmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HDFn cells and HeLa cells; Hela, HepG2, Rat and Mouse liver cell lysates.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
Notes
IP
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
Application notes
Is unsuitable for WB.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
    Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
    ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
    Note: the target protein locates to the mitochondria.
  • Immunoprecipitation - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
    Immunoprecipitation - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)

    Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
    Staining of Aconitase in HeLa, HepG2, Rat liver homogenate (RLH), and Mouse liver homogenate (MLH) lysates immunoprecipitated using ab110320.

  • Flow Cytometry - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
    Flow Cytometry - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
    ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

References (0)

Publishing research using ab110320? Please let us know so that we can cite the reference in this datasheet.

ab110320 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Hello, I'm on the search for an antibody against aconitase2 that can be used for IP in MEF cells, and I see that you have two possible candidates, ab110321 and ab110320. I'm wondering if there's any major differences between these two, and which you would recommend for my use. Thanks so much. Sincerely,

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Answer

Thank you for contacting us. Both ACO2 antibodies cross-react with mouse, however ab110320 is more suitable for immunoprecipitation. Ab110321 is a good WB antibody. In this particular case, I would recommend using ab110320 then. I hope this information is helpful. Please do not hesitate to contact us if you need any more advice or information.

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