For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    acth-antibody-57-n-terminal-ab233954.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmitter Neuropeptides Hormones
Share by email
Recombinant

Recombinant Anti-ACTH antibody [57] - N-terminal (ab233954)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)
  • Anti-ACTH antibody [57] - N-terminal (ab233954)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [57] to ACTH - N-terminal
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Primary
HRP Anti-ACTH antibody [6Y8] - N-terminal (ab187929)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Pair
Product image
Human ACTH Antibody Pair - BSA and Azide free (ab270371)

View more associated products

Overview

  • Product name

    Anti-ACTH antibody [57] - N-terminal
    See all ACTH primary antibodies
  • Description

    Mouse monoclonal [57] to ACTH - N-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human ACTH (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P01189

  • Positive control

    • IHC-P: Human, mouse and rat pituitary gland tissues.
  • General notes

    This product was switched from a hybridoma to recombinant production format on 25th November 2021.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    57
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Hypothalamic pituitary adrenal axis
    • Cancer
    • Tumor biomarkers
    • Hormones
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-ACTH antibody [57] - BSA and Azide free (ab212736)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab233954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Recommend 1:5000 for human species and 1:20, 000 for mouse and rat species

 

Notes
IHC-P
1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Recommend 1:5000 for human species and 1:20, 000 for mouse and rat species

 

Target

  • Relevance

    ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 5443 Human
    • Entrez Gene: 18976 Mouse
    • Entrez Gene: 24664 Rat
    • Omim: 176830 Human
    • SwissProt: P01189 Human
    • SwissProt: P01193 Mouse
    • SwissProt: P01194 Rat
    • Alternative names

      • ACTH antibody
      • Adrenocorticotropic hormone antibody
      • Adrenocorticotropin antibody
      • Alpha melanocyte stimulating hormone antibody
      • alpha-MSH antibody
      • alphaMSH antibody
      • Beta LPH antibody
      • Beta melanocyte stimulating hormone antibody
      • Beta-endorphin antibody
      • beta-MSH antibody
      • CLIP antibody
      • Corticotropin antibody
      • Corticotropin lipotropin antibody
      • Corticotropin-like intermediary peptide antibody
      • Gamma LPH antibody
      • gamma-MSH antibody
      • Lipotropin beta antibody
      • Lipotropin gamma antibody
      • Lipotropin, included antibody
      • LPH antibody
      • Melanocyte-stimulating hormone, included antibody
      • Melanotropin alpha antibody
      • Melanotropin beta antibody
      • Melanotropin gamma antibody
      • Melanotropin, included antibody
      • Met-enkephalin antibody
      • MSH antibody
      • NPP antibody
      • Opiomelanocortin prepropeptide antibody
      • POC antibody
      • POMC antibody
      • Pomc-1 antibody
      • Pomc1 antibody
      • Pomc2 antibody
      • Pro ACTH endorphin antibody
      • Pro opiomelanocortin antibody
      • Pro-opiomelanocortin-alpha antibody
      • Proopiomelanocortin antibody
      • Proopiomelanocortin preproprotein antibody
      • Tetracosactide antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)

      Immunohistochemical analysis of Human pituatary gland tissue stained for ACTH using ab233954 at 1/5000 dilution. Positive staining on human pituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)

      Immunohistochemical analysis of Mouse pituatary gland tissue stained for ACTH using ab233954 at 1/20000 dilution. Positive staining on mouse pituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (ab233954)

      Immunohistochemical analysis of Rat pituatary gland tissue stained for ACTH using ab233954 at 1/20000 dilution. Positive staining on rat pituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Anti-ACTH antibody [57] - N-terminal (ab233954)
      Anti-ACTH antibody [57] - N-terminal (ab233954)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab233954? Please let us know so that we can cite the reference in this datasheet.

    ab233954 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab233954.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.