Anti-Actin antibody [5J11] (ab190301)
Key features and details
- Mouse monoclonal [5J11] to Actin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Actin antibody [5J11]
See all Actin primary antibodies -
Description
Mouse monoclonal [5J11] to Actin -
Host species
Mouse -
Specificity
ab190301 binds to all six isotypes of mammalian actin. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Orangutan -
Immunogen
Full length native protein (purified) corresponding to Cow Actin aa 1 to the C-terminus. derived from bovine brain preparation.
Database link: P68138 -
Positive control
- WB: Rat and mouse brain lysate; NIH/3T3, HEK-293, HeLa and SH-SY5Ycell lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.98% PBS, 49.98% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5J11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/1000.
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WB |
1/1000. Predicted molecular weight: 42 kDa.
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Notes |
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ICC/IF
1/500 - 1/1000. |
WB
1/1000. Predicted molecular weight: 42 kDa. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
- SwissProt: P68135 Rabbit
- SwissProt: P68136 Rat
see all -
Alternative names
- a actin antibody
- a-actin antibody
- ACTA antibody
see all
Images
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All lanes : Anti-Actin antibody [5J11] (ab190301) at 1/1000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Predicted band size: 42 kDaThe same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells (green).
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Immunofluorescent analysis of HeLa cells labeling Actin with ab190301 at 1/1000 dilution (green) and chicken polyclonal antibody to Vimentin (red). The blue stain reveals DNA in the nuclei of these cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab190301 has been referenced in 3 publications.
- Deissler HL et al. Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A165 No Longer Depends on the Growth Factor's Presence. Biomolecules 12:N/A (2022). PubMed: 35625661
- Busch C et al. Type of culture medium determines properties of cultivated retinal endothelial cells: induction of substantial phenotypic conversion by standard DMEM. Heliyon 7:e06037 (2021). PubMed: 33521368
- Jäckle A et al. Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure. J Diabetes Res 2020:2450781 (2020). PubMed: 32566677