Anti-Actin antibody [AC-40] (ab11003)

Thank you for contacting us.

I would not recommend adding SDS or a reducing agent to the sample prior to coating, as this may interfere with the coating efficiency. It is possible that by boiling the protein for 5-10 minutes, the heat denaturation may be enough to expose the epitope that is being recognized by this antibody in WB.

This antibody is designed for detection of beta actin, not skeletal muscle actin. The homology between rabbit skeletal muscle actin (http://www.uniprot.org/uniprot/P68135)and the immunogen for this antibody is only 64%. Using an antibody directed against a more conserved region of actin, such as ab11003, may give better results. This antibody shows 100% homology with the above mentioned rabbit sequence.

I hope this helps, please let me know if you need any additional information or assistance.

The new lot of ab11003 is currently in transit from the production lab to our stock and there is no lot number allocated yet, but I can confirm that this new lot is different from the lot your customer has received originally.

I will send you the lot number as soon as it is created.

We do not have set expiration dates for our products.Aliquotted, not diluted and stored at -20ºC, antibodies, includingab11003is stable for up to 5 years. Up to 10 years when stored at -80ºC. It is very importantto avoid repeated freeze / thaw cycles.We guarantee all of our products to work for at least 120 days from the date of purchase when stored correctly.For more information on antibody storage and stability, please visit our Antibody Storage Guide :https://www.abcam.com/index.html?pageconfig=resource&rid=10795 .

Thank you for your reply.

Unfortunately we do not have any other lot of ab11003 in stock.

Would your customer be interested in trying an alternative anti-actin from the following list (excluding ab11003 of course) : https://www.abcam.com/index.html?pageconfig=searchresults&search=Actin&pt=1&sk=conj&sv=0&sn=Unconjugated&l=2&fViewMore=1

Thank you.

Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem.

I have looked through your protocol, which looks fine to me. It seems that you have received a faulty tube or lot of ab11003. I appreciate the time that has been spent on these experiments and would be pleased to arrange in compensation a credit note or areplacement with an alternative anti-actin from our catalog. The link to thelist of Abcamanti-actin antibodies is : https://www.abcam.com/index.html?pageconfig=searchresults&search=Actin&pt=1&sk=conj&sv=0&sn=Unconjugated&l=2&fViewMore=1

I look forward to hearing from you with details of how you andyour customerwould like to proceed.

Thank you for contacting us.

I am sorry to hear your customeris experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Thank you for your enquiry. Ab8226 does not cross-react with ADULT cardiac, smooth, or skeletal muscle actin. Myoblasts are immature muscle cells and looking through the literature it does seem that some people use actin as a loading control. Your customer may want to try a tubulin loading control. However, I do not want to recommend a product that is unsuitable and suggest that you refer to the latest literature in this field.