Anti-Actin antibody [AC-40] (ab11003)
Key features and details
- Mouse monoclonal [AC-40] to Actin
- Suitable for: ELISA, IHC-P, IHC-Fr, ICC/IF, WB, IHC-FoFr, Dot blot
- Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp, Snail
- Isotype: IgG2a
Overview
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Product name
Anti-Actin antibody [AC-40]
See all Actin primary antibodies -
Description
Mouse monoclonal [AC-40] to Actin -
Host species
Mouse -
Tested applications
Suitable for: ELISA, IHC-P, IHC-Fr, ICC/IF, WB, IHC-FoFr, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp, Snail -
Immunogen
Synthetic peptide within Human Actin aa 365-375 (C terminal). The exact sequence is proprietary.
Sequence:SGPSIVHRKCF
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Epitope
Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms. -
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
AC-40 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | (1) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
IHC-FoFr |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
IHC-FoFr
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 421534 Chicken
- Entrez Gene: 281592 Cow
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 100154254 Pig
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68139 Chicken
see all -
Alternative names
- a actin antibody
- a-actin antibody
- ACTA antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab11003 has been referenced in 49 publications.
- Martins CS et al. Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells. J Cell Biol 222:N/A (2023). PubMed: 36562751
- Chong WC et al. Atypical Teratoid Rhabdoid Tumours Are Susceptible to Panobinostat-Mediated Differentiation Therapy. Cancers (Basel) 13:N/A (2021). PubMed: 34680294
- Yu Q et al. MDA5 disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA. Nat Commun 12:6668 (2021). PubMed: 34795277
- Jäckle A et al. Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure. J Diabetes Res 2020:2450781 (2020). PubMed: 32566677
- Pang N et al. Endothelial cell-derived CCL15 mediates the transmigration of fibrocytes through the CCL15-CCR1 axis in vitro. Mol Med Rep 22:5339-5347 (2020). PubMed: 33174007