Recombinant Anti-Actin antibody [EPR16769] (ab179467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16769] to Actin
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Chicken, Human
Related conjugates and formulations
Overview
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Product name
Anti-Actin antibody [EPR16769]
See all Actin primary antibodies -
Description
Rabbit monoclonal [EPR16769] to Actin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 293T, C6, RAW 264.7, PC-12, NIH/3T3 and UMNSAH/DF-1 whole cell lysates; human skeletal muscle, fetal spleen, fetal brain, fetal heart, fetal kidney and cardiac muscle tissue lysates; mouse and rat brain, heart, kidney and spleen tissue lysates. IHC-P: Human prostate hyperplasia, mouse skeletal muscle, and rat skeletal muscle tissues. ICC/IF: NIH/3T3 cells. IP: NIH/3T3 whole cell extract. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16769 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Actin antibody [EPR16769] (ab206277)
- Alexa Fluor® 647 Anti-Actin antibody [EPR16769] (ab206278)
- HRP Anti-Actin antibody [EPR16769] (ab207674)
- Alexa Fluor® 405 Anti-Actin antibody [EPR16769] (ab207900)
- Alexa Fluor® 555 Anti-Actin antibody [EPR16769] (ab208080)
- Anti-Actin antibody [EPR16769] - BSA and Azide free (ab219733)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/70.
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WB | (8) |
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF | (3) |
1/50.
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IP |
1/40.
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Notes |
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Flow Cyt (Intra)
1/70. |
WB
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50. |
IP
1/40. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 421534 Chicken
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68139 Chicken
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
see all -
Alternative names
- a actin antibody
- a-actin antibody
- ACTA antibody
see all
Images
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All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on smooth muscle cells is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling Actin with Purified ab179467 at 1:100 dilution (6.98 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 dilution (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelia cell) cells labeling Actin with Purified ab179467 at 1:100 dilution ( 6.98 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 dilution (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling Actin with ab179467 at 1/50 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/200 dilution (green). Cytoplasm staining on NIH/3T3 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse Alexa Fluor® 594 secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab179467 at 1/50 dilution followed by ab150120 (goat anti-mouse Alexa Fluor® 594 secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (goat anti-rabbit Alexa Fluor® 488 (IgG H&L) at 1/200 dilution. -
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Actin with purified ab179467 at 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human fetal spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/5000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : Human fetal spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilution
Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates)
Lane 2 : Human cardiac muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Actin was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab179467 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab179467 at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.
Lane 1: NIH/3T3 whole cell extract.
Lane 2: PBS instead of NIH/3T3 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (277)
ab179467 has been referenced in 277 publications.
- Wang Z et al. The Role of EZH2 Inhibitor, GSK-126, in Seizure Susceptibility. J Mol Neurosci 71:556-564 (2021). PubMed: 32772228
- Wen Y et al. Downregulation of lncRNA FIRRE relieved the neuropathic pain of female mice by suppressing HMGB1 expression. Mol Cell Biochem 476:841-852 (2021). PubMed: 33151463
- Tong L et al. circ_100984-miR-432-3p axis regulated c-Jun/YBX-1/ß-catenin feedback loop promotes bladder cancer progression. Cancer Sci 112:1429-1442 (2021). PubMed: 33314480
- Feng R et al. Upregulated microRNA-132 in T helper 17 cells activates hepatic stellate cells to promote hepatocellular carcinoma cell migration in vitro. Scand J Immunol 93:e13007 (2021). PubMed: 33264420
- Qiu X et al. Metformin alleviates ß-glycerophosphate-induced calcification of vascular smooth muscle cells via AMPK/mTOR-activated autophagy. Exp Ther Med 21:58 (2021). PubMed: 33365058