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  1. Link

    products/primary-antibodies/actin-antibody-epr16769-ab179467.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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RecombinantRabMAb

Recombinant Anti-Actin antibody [EPR16769] (ab179467)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (11) Submit a question References (277)

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Western blot - Anti-Actin antibody [EPR16769] (ab179467)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)
  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)
  • Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16769] (ab179467)
  • Western blot - Anti-Actin antibody [EPR16769] (ab179467)
  • Western blot - Anti-Actin antibody [EPR16769] (ab179467)
  • Western blot - Anti-Actin antibody [EPR16769] (ab179467)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
  • Immunoprecipitation - Anti-Actin antibody [EPR16769] (ab179467)
  • Anti-Actin antibody [EPR16769] (ab179467)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16769] to Actin
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Chicken, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free HRP

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Overview

  • Product name

    Anti-Actin antibody [EPR16769]
    See all Actin primary antibodies
  • Description

    Rabbit monoclonal [EPR16769] to Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T, C6, RAW 264.7, PC-12, NIH/3T3 and UMNSAH/DF-1 whole cell lysates; human skeletal muscle, fetal spleen, fetal brain, fetal heart, fetal kidney and cardiac muscle tissue lysates; mouse and rat brain, heart, kidney and spleen tissue lysates. IHC-P: Human prostate hyperplasia, mouse skeletal muscle, and rat skeletal muscle tissues. ICC/IF: NIH/3T3 cells. IP: NIH/3T3 whole cell extract. Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16769
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Isotype/Loading Controls
    • Loading Controls
    • Actin
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Actin antibody [EPR16769] (ab206277)
    • Alexa Fluor® 647 Anti-Actin antibody [EPR16769] (ab206278)
    • HRP Anti-Actin antibody [EPR16769] (ab207674)
    • Alexa Fluor® 405 Anti-Actin antibody [EPR16769] (ab207900)
    • Alexa Fluor® 555 Anti-Actin antibody [EPR16769] (ab208080)
    • Anti-Actin antibody [EPR16769] - BSA and Azide free (ab219733)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Actin protein (Tagged) (ab235861)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/70.
WB (8)
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (3)
1/50.
IP
1/40.
Notes
Flow Cyt (Intra)
1/70.
WB
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50.
IP
1/40.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 421534 Chicken
    • Entrez Gene: 58 Human
    • Entrez Gene: 11459 Mouse
    • Entrez Gene: 29437 Rat
    • Omim: 102610 Human
    • SwissProt: P68139 Chicken
    • SwissProt: P68133 Human
    • SwissProt: P68134 Mouse
    • SwissProt: P68136 Rat
    • Unigene: 1288 Human
    • Unigene: 214950 Mouse
    • Unigene: 82732 Rat
    see all
  • Alternative names

    • a actin antibody
    • a-actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Images

  • Western blot - Anti-Actin antibody [EPR16769] (ab179467)
    Western blot - Anti-Actin antibody [EPR16769] (ab179467)
    All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate
    Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)

    Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on smooth muscle cells is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)
    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling Actin with Purified ab179467 at 1:100 dilution (6.98 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 dilution (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)
    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelia cell) cells labeling Actin with Purified ab179467 at 1:100 dilution ( 6.98 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 dilution (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)
    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (ab179467)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling Actin with ab179467 at 1/50 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/200 dilution (green). Cytoplasm staining on NIH/3T3 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse Alexa Fluor® 594 secondary) at 1/500 dilution (red).

    The negative controls are as follows;
    1. ab179467 at 1/50 dilution followed by ab150120 (goat anti-mouse Alexa Fluor® 594 secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (goat anti-rabbit Alexa Fluor® 488 (IgG H&L) at 1/200 dilution.

  • Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16769] (ab179467)
    Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16769] (ab179467)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Actin with purified ab179467 at 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Western blot - Anti-Actin antibody [EPR16769] (ab179467)
    Western blot - Anti-Actin antibody [EPR16769] (ab179467)
    All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates
    Lane 3 : Human skeletal muscle tissue lysate
    Lane 4 : Human fetal spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Actin antibody [EPR16769] (ab179467)
    Western blot - Anti-Actin antibody [EPR16769] (ab179467)
    All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/5000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal kidney tissue lysate
    Lane 4 : Human fetal spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Actin antibody [EPR16769] (ab179467)
    Western blot - Anti-Actin antibody [EPR16769] (ab179467)
    All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilution

    Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates)
    Lane 2 : Human cardiac muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [EPR16769] (ab179467)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Actin antibody [EPR16769] (ab179467)
    Immunoprecipitation - Anti-Actin antibody [EPR16769] (ab179467)

    Actin was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab179467 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab179467 at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.

    Lane 1: NIH/3T3 whole cell extract.
    Lane 2: PBS instead of NIH/3T3 whole cell extract.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Actin antibody [EPR16769] (ab179467)
    Anti-Actin antibody [EPR16769] (ab179467)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (277)

Publishing research using ab179467? Please let us know so that we can cite the reference in this datasheet.

ab179467 has been referenced in 277 publications.

  • Wang Z  et al. The Role of EZH2 Inhibitor, GSK-126, in Seizure Susceptibility. J Mol Neurosci 71:556-564 (2021). PubMed: 32772228
  • Wen Y  et al. Downregulation of lncRNA FIRRE relieved the neuropathic pain of female mice by suppressing HMGB1 expression. Mol Cell Biochem 476:841-852 (2021). PubMed: 33151463
  • Tong L  et al. circ_100984-miR-432-3p axis regulated c-Jun/YBX-1/ß-catenin feedback loop promotes bladder cancer progression. Cancer Sci 112:1429-1442 (2021). PubMed: 33314480
  • Feng R  et al. Upregulated microRNA-132 in T helper 17 cells activates hepatic stellate cells to promote hepatocellular carcinoma cell migration in vitro. Scand J Immunol 93:e13007 (2021). PubMed: 33264420
  • Qiu X  et al. Metformin alleviates ß-glycerophosphate-induced calcification of vascular smooth muscle cells via AMPK/mTOR-activated autophagy. Exp Ther Med 21:58 (2021). PubMed: 33365058
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-Actin antibody [EPR16769]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
10 µg
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Łukasz Witucki

Verified customer

Submitted Apr 18 2022

Western blot abreview for Anti-Actin antibody [EPR16769]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK2 cell line)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
MDCK2 cell line
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Sep 30 2021

Western blot abreview for Anti-Actin antibody [EPR16769]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat brain)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
rat brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Sep 30 2021

Western blot abreview for Anti-Actin antibody [EPR16769]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse whole brain)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Mouse whole brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Jan 27 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Actin antibody [EPR16769]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human primary dermal fibroblasts)
Permeabilization
Yes - 0.5% TX100
Specification
Human primary dermal fibroblasts
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Nov 28 2019

Western blot abreview for Anti-Actin antibody [EPR16769]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Huh7, hepatocyte-derived carcinoma cell line)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Huh7, hepatocyte-derived carcinoma cell line
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Oct 26 2019

Western blot abreview for Anti-Actin antibody [EPR16769]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Gerbil Cell lysate - whole cell (Mongolian gerbil brain)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
25 µg
Specification
Mongolian gerbil brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Oct 25 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Actin antibody [EPR16769]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MC3T3)
Permeabilization
Yes - NP40
Specification
MC3T3
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 12 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Actin antibody [EPR16769]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 29 2019

Western blot abreview for Anti-Actin antibody [EPR16769]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 10 2019

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