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    products/primary-antibodies/active-yap1-antibody-epr19812-ab205270.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Src Family
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-active YAP1 antibody [EPR19812] (ab205270)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (22) Submit a question References (43)

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Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)
  • Anti-active YAP1 antibody [EPR19812] (ab205270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19812] to active YAP1
  • Suitable for: ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
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Alexa Fluor® 647 Anti-active YAP1 antibody [EPR19812] (ab225440)

View more associated products

Overview

  • Product name

    Anti-active YAP1 antibody [EPR19812]
    See all active YAP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19812] to active YAP1
  • Host species

    Rabbit
  • Specificity

    ab205270 is specific to the active (non-phosphorylated) form of YAP1.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293A cell lysate serum starved overnight, then 10% FBS was added to medium for 1 hour; 293A cell lysate serum starved overnight and then treated with Lambda phosphatase lysate. Human kidney and skin lysates and mouse testis and skin lysates. HaCaT whole cell lysate, 293A cell lysate. HeLa and NIH/3T3 treated with Calyculin A. IHC-P: Human breast and breast cancer tissues; Mouse skin tissue. ICC/IF: 293A cell line serum starved overnight, then 10% FBS was added to medium for 1 hour; 293A cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19812
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Other

Associated products

  • Alternative Versions

    • Anti-active YAP1 antibody [EPR19812] - BSA and Azide free (ab223126)
    • Alexa Fluor® 647 Anti-active YAP1 antibody [EPR19812] (ab225440)
    • Alexa Fluor® 488 Anti-active YAP1 antibody [EPR19812] (ab225441)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
1/500.
IHC-P (10)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (2)
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 54 kDa).
Notes
ICC/IF
1/500.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 54 kDa).

Target

  • Target information above from: UniProt accession P46937 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10413 Human
    • Entrez Gene: 22601 Mouse
    • SwissProt: P46937 Human
    • SwissProt: P46938 Mouse
    • Unigene: 503692 Human
    • Alternative names

      • Protein yorkie homolog antibody
      • Transcriptional coactivator YAP1 antibody
      • YAP1 antibody
      • YAP1_HUMAN antibody
      • Yes-associated protein 1 antibody
      • Yes-associated protein YAP65 homolog antibody
      see all

    Images

    • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      All lanes : Anti-active YAP1 antibody [EPR19812] (ab205270) at 1 µg/ml

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : YAP1 knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 54 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab205270 observed at 54 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab205270 was shown to recognize active YAP1 in wild-type HAP1 cells as signal was lost at the expected MW in active YAP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and active YAP1 knockout samples were subjected to SDS-PAGE. Ab205270 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)

      Immunohistochemical analysis of paraffin-embedded human breast tissue labeling active YAP1 with ab205270 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Mainly nuclear staining on human breast is observed [PMID: 18617895].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)

      Ab205270 staining active YAP1 in HUVEC (human umbilical vein endothelial cell) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). The cells were fixed 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution. An Alexa Fluor® 488 Goat anti-Rabbit was used as a secondary antibody at 1:1000 dilution. DAPI was used as a nuclear counter stain. Confocal image showing nuclear and cytoplasmic staining in HUVEC cells.

    • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton/PBS permeabilized (RT, 5 mins) 293A (Human epithelial cell line from embryonic kidney transformed with sheared human adenovirus type 5 DNA) cells labeling active YAP1 with ab205270 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 594) secondary antibody at 1/1000 dilution.

      The images showed weak staining on 293A cells under serum starvation overnight. After 10% FBS was added to the medium for 1h, the nuclear staining was increased.

      The data was kindly provided by our collaborator Dr. Bin Zhao (Zhejiang University).

      The nuclear counterstain is DAPI (blue). Counterstained with Phalloidin-technology® Alexa Fluor 488 at 1/1000 dilution.

    • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      All lanes : Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

      Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
      Lane 2 : HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg
      Lane 3 : HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 54 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?


      Exposure time: 80 seconds


      Blocking buffer: 5% NFDM/TBST.

    • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      All lanes : Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

      Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
      Lane 2 : NIH/3T3 treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg
      Lane 3 : NIH/3T3 treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 54 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      Blocking buffer: 5% NFDM/TBST.

    • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      All lanes : Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

      Lane 1 : 293A cells under serum starvation overnight lysate
      Lane 2 : 293A cell lysate under serum starvation overnight, then 10% FBS was added to medium for 1 hour

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Serum starvation induces active YAP1 Ser127 phosphorylation. The level of active YAP1 protein is inversely proportional to p-YAP1 Ser127 level (PMID: 22884261).

    • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      All lanes : Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

      Lane 1 : Human kidney lysate
      Lane 2 : Human skin lysate
      Lane 3 : HaCaT (Human keratinocyte cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
      Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1/2: 8 seconds; Lane 3: 30 seconds.

    • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      All lanes : Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

      Lane 1 : Mouse testis lysate
      Lane 2 : Mouse skin lysate
      Lane 3 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 10 seconds; Lane 2: 8 seconds; Lane 3: 3 minutes.

    • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      All lanes : Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

      Lane 1 : 293A cell lysate serum starved overnight and then 10% FBS added to the medium for 1 hour
      Lane 2 : 293A cell lysate serum starved overnight
      Lane 3 : 293A cell lysate serum starved overnight and then treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : donkey anti-rabbit at 1/1000 dilution

      Predicted band size: 54 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% BSA/TBST.

      The two panels for pYAPS127 were just shorter and longer exposure.

      The data was kindly provided by our collaborator Dr. Bin Zhao (Zhejiang University).

    • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)
      All lanes : Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

      Lane 1 : 293A cell lysate
      Lane 2 : YAP/TAZ knockout 293A cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : donkey anti-rabbit at 1/1000 dilution

      Predicted band size: 54 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% BSA/TBST.

      The data was kindly provided by our collaborator Dr. Bin Zhao (Zhejiang University).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling active YAP1 with ab205270 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nuclear and cytoplasmic staining on human breast cancer is observed [PMID: 24559095].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)

      Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling active YAP1 with ab205270 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Mainly nuclear staining on mouse skin is observed [PMID: 21610251].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton/PBS permeabilized (RT, 5 mins) 293A (Human epithelial cell line from embryonic kidney transformed with sheared human adenovirus type 5 DNA) cells labeling active YAP1 with ab205270 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 594) secondary antibody at 1/1000 dilution.

      The images showed nuclear staining on 293A cells, and background staining on YAP/TAZ knockout 293A cells.

      The data was kindly provided by our collaborator Dr. Bin Zhao (Zhejiang University).

      The nuclear counterstain is DAPI (blue). Counterstained with Phalloidin-technology® Alexa Fluor 488 at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)

      Immunohistochemical analysis of paraffin-embedded human liver tissue labeling active YAP1 with ab205270 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative control: no staining on human liver [PMID:17974916].

      Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-active YAP1 antibody [EPR19812] (ab205270)
      Anti-active YAP1 antibody [EPR19812] (ab205270)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (43)

    Publishing research using ab205270? Please let us know so that we can cite the reference in this datasheet.

    ab205270 has been referenced in 43 publications.

    • Muhammad JS  et al. Estrogen-induced hypomethylation and overexpression of YAP1 facilitate breast cancer cell growth and survival. Neoplasia 23:68-79 (2021). PubMed: 33242831
    • Zhou J  et al. The YAP/HIF-1a/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation. Cell Biosci 11:84 (2021). PubMed: 33980319
    • Liu S  et al. Intra-platelet serotonin and YAP contributed to poor prognosis of hepatocellular carcinoma. Life Sci 270:119140 (2021). PubMed: 33524420
    • Amrenova A  et al. Cell competition between anaplastic thyroid cancer and normal thyroid follicular cells exerts reciprocal stress response defining tumor suppressive effects of normal epithelial tissue. PLoS One 16:e0249059 (2021). PubMed: 33793628
    • Chu Y  et al. Adipose-derived mesenchymal stem cells induced PAX8 promotes ovarian cancer cell growth by stabilizing TAZ protein. J Cell Mol Med 25:4434-4443 (2021). PubMed: 33830648
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-10 of 22 Abreviews

    Immunocytochemistry abreview for Anti-active YAP1 antibody [EPR19812]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Kerantinocytes)
    Permeabilization
    Yes - methanol
    Specification
    Kerantinocytes
    Blocking step
    Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 21 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-active YAP1 antibody [EPR19812]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skeletal muscle)
    Permeabilization
    Yes - TritonX (0.1%)
    Specification
    Skeletal muscle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 28 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-YAP1 antibody [EPR19812]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 ab93678
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 29 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-YAP1 antibody [EPR19812]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (breast cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9 Tris EDTA
    Permeabilization
    No
    Specification
    breast cancer
    Blocking step
    Zytomed Systems Antibody Diluent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Submitted Jun 11 2020

    Western blot abreview for Anti-YAP1 antibody [EPR19812]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon cancer cell line)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    50 µg
    Treatment
    chemical inhibitor
    Specification
    colon cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Submitted Mar 19 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-YAP1 antibody [EPR19812]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 ab93678
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-YAP1 antibody [EPR19812]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 ab93678
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-YAP1 antibody [EPR19812]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 ab93678
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-YAP1 antibody [EPR19812]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 ab93678
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-YAP1 antibody [EPR19812]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoids)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney H9 Organoids
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    1-10 of 22 Abreviews

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