Recombinant Anti-ADAM10 antibody [EPR23491-74] (ab252234)
![Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody [EPR23491-74] (ab252234)](https://www.abcam.com/ps/products/252/ab252234/Images/ab252234-2-anti-adam10-antibody-epr2349174-immunocytochemistry-hct-116.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody [EPR23491-74] (ab252234)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23491-74] to ADAM10
- Suitable for: ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ADAM10 antibody [EPR23491-74]
See all ADAM10 primary antibodies -
Description
Rabbit monoclonal [EPR23491-74] to ADAM10 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: LNCaP and HCT116 cells. Flow Cyt: HCT116 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23491-74 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
ICC/IF
1/100. |
Target
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Function
Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. -
Tissue specificity
Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi. - Information by UniProt
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Database links
- Entrez Gene: 102 Human
- Omim: 602192 Human
- SwissProt: O14672 Human
- Unigene: 578508 Human
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Alternative names
- A disintegrin and metalloprotease domain 10 antibody
- A disintegrin and metalloproteinase domain 10 antibody
- AD 10 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody [EPR23491-74] (ab252234)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 cells labelling ADAM10 with ab252234 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in HCT 116 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Low expression control: HeLa (PMID: 23091066).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody [EPR23491-74] (ab252234)](https://www.abcam.com/ps/products/252/ab252234/Images/ab252234-1-anti-adam10-antibody-epr2349174-immunocytochemistry-lncap.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody [EPR23491-74] (ab252234)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP cells labelling ADAM10 with ab252234 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in LNCaP cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-ADAM10 antibody [EPR23491-74] (ab252234)](https://www.abcam.com/ps/products/252/ab252234/Images/ab252234-4-benefits-of-recombinant-antibodies.png)
Anti-ADAM10 antibody [EPR23491-74] (ab252234)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab252234 has not yet been referenced specifically in any publications.