Recombinant Anti-ADAM17 antibody [EPR24095-50] (ab307269)
![Flow Cytometry - Anti-ADAM17 antibody [EPR24095-50] (ab307269)](https://www.abcam.com/ps/products/307/ab307269/Images/ab307269-1-anti-adam17-antibody-epr24095-50-flow-cytometry-wild-type-hct116.jpg "Flow Cytometry - Anti-ADAM17 antibody [EPR24095-50] (ab307269)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24095-50] to ADAM17
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ADAM17 antibody [EPR24095-50]
See all ADAM17 primary antibodies -
Description
Rabbit monoclonal [EPR24095-50] to ADAM17 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Wild-type HCT116 cells. Human peripheral blood mononuclear cell (PBMC).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24095-50 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50.
|
| Notes |
|---|
|
Flow Cyt
1/50. |
Target
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Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway. -
Tissue specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
Domain
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6868 Human
- Omim: 603639 Human
- SwissProt: P78536 Human
- Unigene: 404914 Human
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Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
Images
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Flow Cytometry - Anti-ADAM17 antibody [EPR24095-50] (ab307269)
Flow cytometric analysis of Wild-type HCT116 (human colorectal carcinoma epithelial cell, Right) / ADAM17 knockout HCT116 (Left) cells labeling ADAM17 with AB307269 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Positive staining on HCT116 cells (ab266873), while no staining on ADAM17 knockout HCT116 cells (ab255451).
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![Flow Cytometry - Anti-ADAM17 antibody [EPR24095-50] (ab307269)](https://www.abcam.com/ps/products/307/ab307269/Images/ab307269-2-anti-adam17-antibody-epr24095-50-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-ADAM17 antibody [EPR24095-50] (ab307269)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling ADAM17 with AB307269 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.
Cells were stained with rabbit IgG or ab307269. Then stained with anti-CD14 conjugated to BV510.
Secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) used at 1/2000 dilution.
Gated on viable cells.
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![Anti-ADAM17 antibody [EPR24095-50] (ab307269)](https://www.abcam.com/ps/products/307/ab307269/Images/ab307269-3-BenefitsofRecombinantantibodies.jpg)
Anti-ADAM17 antibody [EPR24095-50] (ab307269)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab307269 has not yet been referenced specifically in any publications.