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RecombinantRabMAb

Recombinant Anti-ADH7 antibody [EPR13949] (ab186408)

Reviews (2) Submit a question
Western blot - Anti-ADH7 antibody [EPR13949] (ab186408)
  • Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] (ab186408)
  • Anti-ADH7 antibody [EPR13949] (ab186408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13949] to ADH7
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ADH7 antibody [EPR13949]
    See all ADH7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13949] to ADH7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Common marmoset

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human stomach and fetal liver lysates; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
WB
1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Notes
ICC/IF
1/100.
WB
1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • Relevance

    This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.

  • Cellular localization

    Cytoplasm

  • Database links

    • Alternative names

      • ADH 4 antibody
      • ADH 7 antibody
      • ADH, class IV antibody
      • ADH4 antibody
      • Alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide antibody
      • alcohol dehydrogenase 7 antibody
      • alcohol dehydrogenase class 4 mu/sigma chain antibody
      • Alcohol dehydrogenase class IV mu/sigma chain antibody
      • alcohol dehydrogenase VII antibody
      • class IV sigma 1 alcohol dehydrogenase antibody
      • class IV sigmasigma alcohol dehydrogenase antibody
      • Gastric alcohol dehydrogenase antibody
      • Gastric H(+)/K(+) ATPase subunit alpha antibody
      • Retinol dehydrogenase antibody
      see all

    Images

    • Western blot - Anti-ADH7 antibody [EPR13949] (ab186408)
      Western blot - Anti-ADH7 antibody [EPR13949] (ab186408)
      All lanes : Anti-ADH7 antibody [EPR13949] (ab186408) at 1/1000 dilution

      Lane 1 : Human stomach lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] (ab186408)
      Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] (ab186408)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ADH7 with ab186408 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    • Anti-ADH7 antibody [EPR13949] (ab186408)
      Anti-ADH7 antibody [EPR13949] (ab186408)

    References (0)

    Publishing research using ab186408? Please let us know so that we can cite the reference in this datasheet.

    ab186408 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ADH7 antibody [EPR13949]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Common marmoset Tissue sections (Stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Stomach
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Feb 17 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ADH7 antibody [EPR13949]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Feb 17 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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